%0 Journal Article
%T Identification and Quantification of Nosema Species in Honey Bee Colonies at Malakand Division, Khyber Pakhtunkhwa, Pakistan
%A Shawal Shalmani
%A Abdul Muhsin
%A Mohammad Attaullah
%A Syeda Maria Jan
%A Iqra Hakim
%A Sanaullah Khan
%A Nouman Hussain
%J Entomology and Applied Science Letters
%@ 2349-2864
%D 2025
%V 12
%N 2
%R 10.51847/xmcQQ5jWkH
%P 1-7
%X Honeybees play a crucial role in agriculture and the ecosystem through pollination services. They are susceptible to numerous pathogens. Nosemosis is one of the pathological conditions that obvious repercussions on honeybee colonies and is caused by the microsporidium belonging to the genus-Nosemosis. Nosema spp is an obligatory intracellular, ventricular parasite belonging to the kingdom Fungi. The present study focused on the etiology of Nosemosis in honeybee colonies at Malakand Division. Due to the global prevalence of Nosemosis and the huge extermination of honeybee colonies by Nosemosis, we aimed to evaluate Nosemosis in Malakand Division. Random sampling was carried out from the length and breadth of the Division. During sampling, we collect symptomatic older foraging bees and also cadavers near the boxes. We analyzed the sample slides through 400x magnification under a light microscope and used Giemsa stain to improve contrast. We work for about eight months. All of our summer and autumn samples were negative for Nosema spores, but the spring samples were positive for spores. For the prevalence and abundance of spores, we conducted hemocytometry for positive samples and compared the findings with previous literature. The present study highlights that the prevalence rate of Nosemosis is low in the Malakand Division. Further studies are recommended to evaluate Nosemosis through modern molecular techniques, considering all possible sites of the region spanning over the entire year.
%U https://easletters.com/article/identification-and-quantification-of-nosema-species-in-honey-bee-colonies-at-malakand-division-khyb-r1m7xq5ivnut3iy