%0 Journal Article
%T Morphology and Molecular Study of Cassia angustifolia Vahl. in Saudi Arabia Using RAPD Technique
%A Moodi Saham Alsubeie
%J Entomology and Applied Science Letters
%@ 2349-2864
%D 2022
%V 9
%N 2
%R 10.51847/434ps9amNE
%P 11-16
%X Cassia angustifolia Vahl. is native to Saudi Arabia. This shrub has been used as a laxative and even as a fungicide. The current study was designed to evaluate the morphology and molecular characterization of Cassia angustifolia plants from two areas in Saudi Arabia. Purified genomic DNA was subjected to PCR amplification using random primers (RAPD). The study showed that, all morphological parameters were statistically significant (P ≤ 0.05) when comparing Cassia angustifolia plants from the two areas. The primers screened (APOM3, APOM9, UBC-101, UBC-104) showed amplification in all subjected samples. The sizes of the ladder DNA were 1 kb. In four primers, the 1, 2, 3, and 4 RAPD markers produced 10 bands. The observed bands ranged in size from 100 Pb to 600 Pb, with one to three bands per primer. Ten DNA fragments were polymorphic bands between both two plants were confirmed in phonomertic characters observed in this study. This suggests that cassia angustifolia plants from A and B are related but show morphological variation.
%U https://easletters.com/article/morphology-and-molecular-study-of-cassia-angustifolia-vahl-in-saudi-arabia-using-rapd-technique-zx6jgwtfvbkuiq7