Total phenol content in thrips infested rice leaves of Khasi Hills

Thrips infestation in rice leaves causes a considerable leaf yield loss and quality. An attempt was made to know the changes in secondary metabolites constituents (viz, total phenols) in normal and thrips infested cultivated rice leaves of Mawlai (25º 60’ N 91º 90’ E), and Nongjri (25º 74’ N 91º 90’ E) of Khasi Hill. The amount of total phenols, were analyzed using a spectrophotometric technique, based on Folin-Ciocalteau reagent showed significant variation in phenol content from both the sites as compared to the normal rice leaf. Gallic acid was used as standard compound and the total phenols were expressed as mg GAE/g leaf extract. The total phenols varied from 18.89 ± 0.39 to 28.55 ± 0.98 mg GAE/g leaf extract in normal leaf extracts. A higher phenolic content was found in thrips infested leaf extract varying from 287.29 ± 5.06 to 340.3 ± 0.72 mg GAE/g leaf extract in both the studied sites.

Keywords: Rice leaf, normal, infested, phenolic content, secondary metabolites

P. Bhattacharjee, R. S. Singhal, P. R. Kulkarni, Int J Food Biophys, 2002, 49, 115-123

G. S. Khush, Pl Mol Bio, 2005, 59, 1-6

Food and Agriculture Organization (FAO) (2009-2010), International Exhibition to Promote rice Import & Export, Abu Dhabi National Exhibition Centre, UAE. 2012

L. M. Schoonhoven, T. Jermey, J. J. A van Loon, Insect Plant-biology: From Physiology to Evolution; Chapman and Hall: London, UK. 1998

D. F. Houlihan, J. Zool, 1969, 159, 249–267

G. A. Howe, G. Jander, Annu Rev Plant Biol, 2008, 59, 41-66

J. D. Hare, Annu Rev Entomol, 2011, 56, 161-80

W. Agosta, Bombardier beetle and fever trees: A close up look at chemical warfare and signals in animals and plants. Addison-Wesley, Reading, MA. 1996

M. G. Barbour, J. H. Burk, W. D. Pitts, Terrestrial plant ecology, Second edition, Benjamin/Cummings, Menlo Park, Calif. 1987

R. M. Bostock, Physiol Mol Plant P, 1999, 55, 99-109.

R. M. Bostock, R. Karban, J. S. Thaler, P. D. Weyman, D. Gilchrist, European J Plant Pathol, 2001,107, 103-111

R. Hell, Planta, 1997, 202, 138-148

B. P. H. J. Thomma, K. Eggermont, I. A. M. A. Penninckx, B. Mauch-Mani, R. Vogelsang, B. P. A. Cammue, W. F. Broekaert, Proc Nat Acad Sci, 1998, 95, 15107-15111

P. Vijayan, J. Shockey, C. A. Levesque, R. J. Cook, J. Browse, Proc Nat Acad Sci, 1998, 95, 7209-7214

R. H. Whittaker, P. P. Feeny, Sci, 1971, 171, 757-770

A. Kusnieczyk, P. Winge, H. Midelfart, W. S. Armbruster, J. T. Rossiter, A. M. Bones, J Exp Bot, 2007, 58(10), 2537-2552

N. Dudareva, F. Negre, D. A. Nagegowda, I. Orlova, Crit Rev Plant Sci, 2006, 25, 417-40

G. I. Arimura, K. Matsui, J. Takabayashi, Plant Cell Physiol, 2009, 50, 911-23

M. E. Hanley, B. B. Lamont, M. M. Fairbanks, C. M. Rafferty, Perspec Plant Ecol Evol Syst, 2007, 8,157-78

N. Wuyts, D. De waele, R. Swennen, Plant Physiol Biochem, 2006, 44, 308-314

P. G. Waterman, S. Mole, Analysis of phenolic plant metabolites. Blackwell Scientific Publications, Oxford, UK. 1994

P. F. Feeny, Ecol, 1970, 51, 565-581

P. F. Feeny, Rec. Adv. Phytochem, 1976, 10, 1-40

D. F. Rhoades, R. G. Cates, Rec. Adv. Phytochem, 1976, 10, 168-213

A. Ikonen, , J. Tahvanainen, H. Roininen, Chemo ecol, 2001, 12(3), 125-131

D. R. Johnson, J. J. Kimbrough, M. L. Wall, Coop. Ext. Serv. EL, 1987, 330, 1-15

D. P. Pashley, Entomol, 1988, 71, 227- 233

N. T. Chang, Chinese J. Econ. Entomol, 1986, 6(2), 163-175

D. P. Pashley, Causes of host-associated variation in insect herbivores: an example from fall armyworm., in: Kim, K.C. and B.A. McPheron (eds.), Evolution of insect pests. John Wiley and Sons, Inc., New York. 1993, pp 351-359

D. Classen, J. T. Arnason, J. A. Serratos, J. D. H. Lambert, C. Nozzolillo, B. J. R. Philogene, J. Chem. Ecol, 1990, 16, 301-315

H. Wu, T. Haig, J. Pratley, D. Lemmerle, M. An, J Chem Ecol, 2001, 27, 125-135

S. Amudhan, U. P. Rao, J. S. Bentur, Current Sci, 1999, 76(12), 1577-1580

M. P. Ayers, T. P. Clausen, S. F. MacLean, A. H. Redman, P. B. Reichardt, Ecol, 1997, 78, 1696-1712

R. L. Lindroth, S. S. Peterson, Oecologia , 1988, 75, 185-189

M. Kharbangar, S. R. Hajong, S. Choudhury, J Entomol Zool Stud, 2013, 1(6), 100-104

C. P. Malick and M. B. Singh. In: Plant Enzymology and Histo Enzymology, Kalyani Publishers, New Delhi. 1980, pp 286

D. Dent, Insect pest management, 2nd ed. CAB international, Wallingford, UK. 2000

L. P. Pedigo, Entomology and pest management, 3rd ed. Prentice- Hall, New Jersey. 1999

T. Lewis, Thrips, their biology, ecology and economic importance, Academic Press London and New York. 1973

K. Esau. In: Anatomy of seed plants, New York & London, Wiley. 1961, pp 376

B. K. Salunke, H. M. Kotkar, P. S. Mendki, S. M. Upasani, V. L. Maheshwari, Crop Prot. 2005, 24: 888-893

S. E. Brodeur-Campbell, J.A. Vucetich, D. L. Richter, T. A.Waite, J. N. Rosemier, C. J. Tsai, Environ Entomol, 2006, 35, 1696-1701

F. C. Schroeder, M. L. del Campo, J. B. Grant, D. B. Weibel, S. R. Smedley, K. L. Bolton, J. Meinwald, T. Eisner, Proc Nat Acad Sci, USA, 2006, 103, 15497-15501.

C. M. DeMoraes, M. C. Mescher, J. H. Tumlinson, Nature, 2001, 410, 577-580

D. P. W. Huber, J. Bohlmann, The role of terpene synthases in the direct and indirect defense of conifers against insect herbivory and fungal pathogens, Multigenic and Induced Systemic Resistance in Plants (eds. S. Tuzun and E. Bent), Springer-Verlag, New York. 2006, pp 296-313.

O. Koul, Insect Anrifeedants, CRC Press, Boca Raton, FL. 2005

J. Krasaetep, M. Nakornriab, D. Puangpronpitag, Int J Appl Chem, 2011, 7, 285 – 296

A. Moure, D. Franco, J. Sineiro, H. Domĭngguez, M. J. Nūňez, J. M. Lema, Food Res Int, 2001, 34(2), 103-109

R. Nowak, U. Gawlik-Dziki, Z Naturforsch, 2007, 62, 32- 38