2019 Volume 6 Issue 2

Punica Granatum-Based Green Ethanolic Extract as Highly Effective and Eco-Friendly Larvicide, Repellent against Medically Important Mosqui-to Vectors

M. Baranitharan, V. Tamizhazhagan, K. Kovendan, S. Senthilmurugan

Background & objective: Mosquito vectors are living organisms that can transmit infectious diseases between human from animals to humans. It is bloodsucking insect that ingest disease-producing microorganisms during a blood meal from an infected host. The present investigation discovered that the larvicidal activity of ethanol extract of P. granatum showed most mortality among the opposite crude extracts. Methods: The chemical composition of P. granatum ethanolic extract was analyzed by gas chromatography-mass spectroscopy. A total of twenty five III instar larvae of An. stephensi and Cx. quinquefasciatus were exposed to various concentrations (50-250 ppm) in the laboratory by using the standard protocol described by WHO (2005). The repellent activity of P. granatum chemical compositions tested against 100 blood starved female mosquitoes of An. stephensi and Cx. quinquefasciatus using the protocol of WHO (1996). Results: In GC-MS analysis, a total of seven compounds were identified in the ethanolic extract composition, the main component was Methyl 4-piperidineacetate. Further, the LC50 and LC90 values were found to be 110.36 and 212.28 mg/L against Cx. quinquefasciatus. The repellent activity to be best and therefore the most activity was ascertained at 3.5 mg/cm2 concentration provided 100% protection up to 240 min against Cx. quinquefasciatus. Conclusion: The results clearly show confirmed that the presence of active compounds in leaf of P. granatum.


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Entomology and Applied Science Letters is an international peer reviewed publication which publishes scientific research & review articles related to insects that contain information of interest to a wider audience, e.g. papers bearing on the theoretical, genetic, agricultural, medical and biodiversity issues. Emphasis is also placed on the selection of comprehensive, revisionary or integrated systematics studies of broader biological or zoogeographical relevance. Papers on non-insect groups are no longer accepted. In addition to full-length research articles and reviews, the journal publishes interpretive articles in a Forum section, Short Communications, and Letters to the Editor. The journal publishes reports on all phases of medical entomology and medical acarology, including the systematics and biology of insects, acarines, and other arthropods of public health and veterinary significance.
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