An In Vitro Study to Compare Antibacterial Efficacy of Various Root Canal Irrigants Used In Endodontics

Zeeshan Qamar, Tayyaba Fatima


The aim of the study was to compare antibacterial efficacy of various root canal irrigants used in department of endodontics. One hundred and fifty freshly extracted mandibular & maxillary molars were stored in normal saline at room temperatures .The teeth were deroofed & their wider canals that is distal canal in mandibular molars & palatal canal in maxillary molars were selected. Working lengths of specimens were recorded. All teeth were washed & autoclaved. Widening of canals was done using the K-file with a step back technique and divided into 5 groups (30/group).The teeth were then inoculated with 10 microliter of bacterial suspension of enterococcus faecalis fixed at 0.144 absorbance value (550nm wavelength) and incubated for 48-hrs. There was evident statistically significant difference in the antibacterial efficiency between 5% sodium hypochlorite (NaOCl) (positive control) (Group A) 0.12% chlorhexidine (CHX) (Group B), 2% Iodine (Iodine potassium iodide) (Group C), Sodium Chloride (NaCl) (Group D) and distilled water (Group E) (negative control) (ρ<0.05). Group A where 5ml of 5% NaOCl was used at initial & final rinse as root canal irrigants showed the maximum antibacterial efficiency against streptococci followed by Group B, Group C, Group D & Group E (Control Group).

Key Words: Root canal irrigants, NaOCl, chlorhexidine, NaCl, Iodine

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