The Sensitivity of the Real-time PCR and Nested-PCR for Detection of Coxiella burnetii in Milk Samples

Mojtaba Bonyadian, Hamdollah Moshtaghi, Hamidreza Kazemeini


Coxiella burnetii is the causative agent of Q-fever, a widespread zoonosis. In domestic animals infection remains either asymptomatic or presents as infertility or abortion. Clinical presentation in humans can range from mild flu-like illness to acute pneumonia and hepatitis. In humans serology is the gold standard for diagnosis but is inadequate for early case detection, so real-time PCR and nested-PCR assays were developed in this study to measure amounts of C.burnetii shed in milk. Our study was to assess the sensitivity of the realtime PCR and nested-PCR for detection of Coxiella burnetii in bovine bulk milk samples from dairy herds in 3 provinces (Chaharmahal and Bakhtiari , Isfahan and Yazd) of Iran. In the present study, 300 bulk milk samples from 89 dairy cattle herds were tested for C. burnetii using real-time PCR and nested-PCR assays. The animals which their milk samples collected for this study were clinically healthy. In total, 74 of 300 (24.7%) cow milk samples were positive in real-time PCR assay and 26 of 300 (8.7%) samples were positive in nested-PCR assay. McNemar test shows a significant difference in detection of C. burnetii between real-time PCR and nested-PCR. Also the results of this study indicate those clinically healthy dairy cows are important sources of C. burnetii infection in Iran.

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Maurin M, Raoult D. Q fever. Clin Microbiol Rev 1999; 12: 518–53.

Hirai K, To H. J Vet Med Sci 1998; 60: 781–90.

Babudieri B. Adv Vet Sci 1959; 5:81–182.

Htwe KK, Amano K, Sugiyama Y, Yagami K, Minamoto N, Hashimoto A, et al. Vet Rec 1992; 131:490. [5] Lang GH. Can J Public Health 1988; 79: 56–9.

Wisniewski H, Krumbiegel ER. Arch Environ Health 1970; 21: 58–62.

Lang GH. Coxiellosis (Q fever) in animals. In: T.J. Marrie, editor. Q fever, volI. The disease. Boca Raton (FL): CRC Press, 1990. pp23–48.

McQuiston JH, Childs JE. Vector Borne Zoonotic Dis 2002; 2: 179–91. [9] Hoover TA, Vodkin MH, Williams JC. J Bacteriol 1992; 174: 5540–8.

Willems H, Thiele D, Frolich-Ritter R, Krauss H. J Vet Med B 1994;41: 580–7.

Luoto L. Public Health Rep 1960;75: 135– 40. [12] Biberstein EL, Behymer DE, Bushnell R, Crenshaw G, Riemann HP, Franti CE. Am J Vet Res 1974;12: 1577–2.

Ghalyanchi Langeroudi A, Raees Babakhani N, Zolfaghari MR ,Majidzadeh KA, Morovvati A, Soleimani M, IJVM 2013; 7: 207-211.

Kazar J, Brezina R, Palaova P. Bull WHO 1982;60: 389-394.

Guatteo R, Beaudeau F, Joly A, Seegers H. Zoonoses and Public Health 2007; 54: 191-194.

Arricau-Bouvery N, Rodolakis A .Is Q fever an emerging or reemerging zoonosis? Vet. Res. 2005;36: 327-349.

Zhang GQ, Nguyen SV, To H, Ogawa M, Hotta A, Yamaguchi T, Kim HJ, Fukushi H, Hirai K. J Clin Microbiol 1998; 36: 77-80.

Addo PB . Br. Vet. J 1980; 136: 519-521.

Soliman AK, Botros BA, Watts DM. J. Clin. Microbiol 1992; 30: 1595-1597.

Rodolakis A, Berri M, Héchard C, Caudron C, Souriau A, Bodier CC, Blanchard B, Camuset P, Devillechaise P, Natorp JC, Vadet JP, ArricauBouvery N. J. Dairy Sci 2007;90: 5352-5360.

Berri M, Arricau-Bouvery N, Rodolakis A. PCR-based detection of Coxiella burnetii from clinical samples. In: Sachse K., Frey J. (Eds.), Methods in molecular biology, Humana Press Inc., Totowa, NJ ,2003. pp. 153-161.

Öngör H, Cetinkaya B, Karahan M, Nuri Acik M, Bulut H, Muz A. Vet. Rec 2004; 154: 570-572.

Fretz R, Schaeren W, Tanner M, A. Baumgartner. Int. J. Food Microbiol 2007; 116: 414-418.




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<Entomology+Zoology+Allied Branches>Entomology and Applied Science Letters