Creative Commons License 2017 Volume 4 Issue 3

Determination of Some Heavy Metals and Elements in Honeybee and Honey Samples from Saudi Arabia

Dalal Musleh Aljedani

Honeybees and honey have become important tools for ecotoxicity because of their extraordinary ability to bioaccumulate of mineral element and toxic metals from the environment. The present study aimed to evaluate and detect the mineral element and pollution levels of honeybees and their product, honey, by heavy metals. These minerals include heavy metals; cadmium, chromium, copper, iron, manganese, nickel, lead, zinc) and elements (calcium, potassium, magnesium, sodium). The levels of heavy metal pollution are collected from different regions in Saudi Arabia using foraging bees of Apis mellifera jemenatica. For this purpose, bee and honey samples were collected directly from different geographic regions in Saudi Arabia. The results of this study showed that the highest contamination level among the heavy metals in question was the iron (Fe). In the honey bee samples, the highest Fe concentrations were in Makkah region (8.794) Asir (6.222) Jazan (6.205)Al-Baha(2.088). In the honey samples, the highest Fe concentrations were found in Asir (1.904) Jazan (1.843)Al-Baha(1.340) and Makkah (0.907). In addition, we found that the most concentrated mineral element is potassium (K) in all four agricultural areas from which the samples were collected in this study. The results showed that metal levels are within the limits of international standards  in bee and honey in Saudi Arabia. 


Celli, G., Maccagnani, B., 2003. Honey bees as bioindicators of environmental pollution. Bulletin of Insectology,56:137–139.

Hennessy, S., Downey, G., O’donnell, C.P., 2010 . Attempted confirmation of the provenance of Corsican PDO honey using FT-IR spectroscopy and multivariate data analysis. Journal of Agricultural and Food Chemistry. 58: 9401–9406.

Pohl, P., Sergiel, I., Prusisz, B., 2011. Direct analysis of honey for the total content of Zn and its fractionation forms by means of flame atomic absorption spectrometry with solid phase extraction and ultrafiltration approaches. Food Chemistry, 125: 1504–1509.

Bogdanov, S., 2006. Contaminants of bee products. Apidologie, 37: 1–18.

Ciobanu, O., Rᾰdulescu, H., 2016. Monitoring of heavy metals residues in honey. Research Journal of Agricultural Science, 48 (3):9-13.ref.8.

van der Steen, J. J. M., de Kraker, J., Grotenhuis, T., 2012. Spatial and temporal variation of metal concentrations in adult honeybees (Apis mellifera L.). Environ Monit Assess, 184:4119–4126. https://doi. 10.1007/s10661-011-2248-7.

Giglio, A., Ammendola, A., Battistella, S., Naccarato, A., Pallavicini, A., Simeon, E. Giulianini, P. G., 2017. Apis mellifera ligustica, Spinola 1806 as bioindicator for detecting environmental contamination: a preliminary study of heavy metal pollution in Trieste, Italy. Environmental Science and Pollution Research, 24(1): 659-665.‏

Baroni, M.V., Arrua, C., Nores, M.L., Faye, P., Diaz, M.D.P., Chiabrando, G.A.,2009 . Composition of honey from Cordoba (Argentina): assessment of North/ South provenance by chemometrics. Food Chem,114:727–33.

Nanda, V., Singh, B., Kukreja, V.K., Bawa, A.S., 2009 . Characterization of honey produced from different fruit plants of northern India. Int J Food Sci Technol, 44:2629–36.

uszczak, L., Socha, R., Roznowski, J., Fortuna, T., Nalepka, K., 2009 . Physicochemical properties and quality parameters of herb honeys. Food Chem.113:538–42.

Silva, L.R., Videira, R., Monteiro, A.P., Valentao, P., Andrade, P.B., 2009 . Honey from Luso region (Portugal): physicochemical characteristics and mineral contents. Microchem J, 93:73–77.

Pisani, A., Protano, G., Riccobono, F., 2008 . Minor and trace elements in different honey types produced in Siena county (Italy). Food Chem, 107:1553–60.

Rashed, M.N., Soltan, M.E. 2004 . Major and trace elements in different types of Egyptian mono-floral and non-floral bee honeys. J Food Compos Anal, 17:725–35.

Shaban, N. S., Abdou, K. A., Hassan, N.Y., 2016. Impact of toxic heavy metals and pesticide residues in herbal products. beni-suef university journal of basic and applied sciences 5 : 102–106.

Baker, A.J.M., McGrath, S.P., Sodoli, C.M., Reeves, D.,1994 . The possibility of in situ heavy Res. Conserv, 11: 41–49.

Munõz-Olivas, R., Camara, C., 2001 . Speciation related to human health. In: Ebdon, L., Pitts, L., Cornelis, R., Crew, H., Donard, O.F.X. and Quevauviller, P., editors. Trace element speciation for environment, food and health. UK: The Royal Society of Chemistry,331–353.

Wang, J., Kliks, M.M., Jun, S., Li, Q.X., 2010 . Residues of organochlorine pesticides in honeys from different geographic regions. Food Res Int, 43:2329–34.

Ru, Q.M., Feng, Q., He, J.Z., 2013 . Risk assessment of heavy metals in honey consumed in Zhejiang province, southeastern China. Food Chem Toxicol, 53:256–62.

Leita, L., Muhlbachova, G., Cesco, S., Barbattini, R., Mondini, C.,1996. Investigation on the use of honeybees and honeybee products to assess heavy metals contamination. Environ Monit Assess, 43:1–9.

Perugini, M., Manera, M., Grotta, L., Cesarina Abete, M., Tarasco, R., Amorena, M., 2011 . Heavy Metal (Hg, Cr, Cd, and Pb) Contamination in Urban Areas and Wildlife Reserves: Honeybees as Bioindicators. Biol Trace Elem Res, 140:170–176. .

Devillers, J., Dore, J.C., Marenco, M., Poirier-Duchene, F., Galand, N., Viel, C., 2002 . Chemometrical analysis of 18 metallic and nonmetallic elements found in honeys sold in France. J Agric Food Chem, 50:5998–6007.

Bilandzic, N., Dokic, M., Sedak, M., Kolanovic,B.S.,Varenina, I., Koncurat, A.,2011 . Determination of trace elements in Croatian floral honey originating from different regions. Food Chem,128:1160–4.

Golob, T., Dobersek, U., Kump, P., Necemer, M.,2005. Determination of trace and minor elements in Slovenian honey by total reflection X-ray fluorescence spectroscopy. Food Chem. 91:593–600.

Przybyłowski, P. Wilczyńska, A.(2001). Honey as an environmental marker. Food Chemistry, 74, 289-291.‏

Citak, D., Silici, S., Tuzen, M., Soylak, M., 2012. Determination of toxic and essential elements in sunflower honey from Thrace region, Turkey. Int J Food Sci Technol 47:107–113.

Silici, S., Uluozlu, D.O., Tuzen, M., Soylak, M., 2008. Assessment of trace element levels in rhododendron honeys of Black sea Region, Turkey. Journal of Hazardous Materials, 156: 612–618.

Tuzen, M., Silici, S., Mendil, D., Soylak, M., 2007 . Trace element levels in honeys from different regions of Turkey. Food Chemist,103:325–30.

Erbilir, F., Erdogrul, O., 2005. Determination of heavy metals in honey in Kahramanmaras city, Turkey. Environmental Monitoring and Assessment, 109: 181–187.

Ruschioni, S., Riolo, P., Minuz, R. L., Stefano, M., Cannella, M., Porrini, C., Isidoro, N. (2013). Biomonitoring with honeybees of heavy metals and pesticides in nature reserves of the Marche Region (Italy).Biol Trace Elem Res, 154(2), 226-33.

Komarnicki, G.J.K., 2005 . Lead and cadmium in indoor air and the urban environment. Environ Pollut, 136:47–61.

Fakhimzadeh, K., Lodenius, M.,2000. Heavy metals in Finnish honey, pollen and honey bees. Apiacta. 35: 85–95.

Conti, M.E., Botre, F., 2001 . Honeybees and their products as potential bioindicators of heavy metals contamination. Environmental Monitoring and Assessment, 69: 267–282.

Amin, S., Ali-Akbar, M., Reza, B., Kamran, S., 2012 . Use of honeybees as bio-indicators of environmental pollution in the Kurdistan Province of Iran. Journal of Apicultural Science, 56(2): doi: 10.2478/v10289-012-0026-6.

Porrini, C. Sabatini, A.G., Girotti, S., Ghini, S., Medrzycki, P., Grillenzoni, F., Bortolotti, L., Gattavecchia, E., Celli, G., 2003 . Honeybees and bee products as monitors of the environmental contamination. Apiacta,38:63–70.

Silici, S., Uluozlu, D.O., Tuzen, M., Soylak, M.,2013. Honeybee and honey as monitors for heavy metal contamination near the thermal power plants in Mugla, Turkey. Toxicology and Industrial Health, 1-10 pp. https://doi. 10.1177/0748233713503393. Source: PubMed.

Tabi, J.A., 2015 . Heavy metals and pesticide in honey from the major honey producing forest belts in Ashanti, Brong Ahafo and Western regions of Ghana. A Thesis for the degree of Master of Philosophy (MPhil.) in Analytical Chemistry , Department of Chemistry, College of Science, Kwame Nkrumah University of Science and Technology, KNUST, Kumasi, (71)pp.

Crane, E.,1975 . Honey: A Comprehensive Survey. London, UK: William Heinemann in co-operation with International Bee Research Association.Published by Crane, Russak and Company, Inc., New York, 1975 .From: BWB Antiquarian, Rare, and Collectable(Mishawaka, IN, U.S.A.).

Bogdanov, S., Haldimann, M., Luginbu¨hl, W., Gallmann, P., 2007 .Minerals in honey: environmental, geographical and botanical aspects. Journal of Apicultural Research and Bee World, 46: 269–275.

Stankovska, E., Stafilov, T., Sajn, R., 2008 . Monitoring of trace elements in honey from the Republic Macedonia by atomic absorption spectrometry. Environmental Monitoring and Assessment, 142: 117–126.

Shah, A., Sikandar, F., Ullah, I., Shah, A., Ud-Din Khan, S., Usman Ali Rana, U.A., McCoy, T.,2014 . Spectrophotometric Determination of Trace Elements in various Honey Samples, Collected from different Environments. Journal of Food and Nutrition Research, 2 (9): 532-538. https://doi. 10.12691/jfnr-2-9-1.

Wagoner, K. M., Rueppell, O., 2017 . Effects of steel foundation wire on elemental content and hygienic removal of honey bee (Apis mellifera) brood. Journal of Apicultural Research, 1-8.‏

Abdulkhaliq, A., Swaileh, K. M.,2017 . Physico-chemical properties of multi-floral honey from the West Bank, Palestine. International Journal of Food Properties, 20(2): 447-454.

Kadri, S. M., Zaluski, R., de Oliveira Orsi, R., 2017 . Nutritional and mineral contents of honey extracted by centrifugation and pressed processes. Food Chemistry,218: 237-241.

Spodniewska, A., 2007 . Lead and cadmium content in bees from apiaries of Warmia and Mazury province. Veterinary Medicine, 63: 736.

Costa-Silva, F., Maia, M., Matos, C.C., Calcada, E., Nunes, F.M., 2011. Selenium content of Portuguese unifloral honeys. Journal of Food Composition and Analysis, 24: 351–355.

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