Entomology and
Applied Science Letters
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Creative Commons License 2016 Volume 3 Issue 3 sp

Trace coliform in drinking water using polymerase chain reaction

Ghodous Fathi, Farzaneh Janmahammadi, Daem Roshani, Kayvan Farahmandi
Abstract

Microbial methods for identification of indicative coliforms of drinking water contamination have attracted the attention of scientific societies worldwide. Microbial methods, which lacks the defects and flaws of the previous methods, can be used as a suitable alternative in vitro. In this study, the PCR amplification of the LacZ gene was used to detect and identify coliforms in drinking water resources of villages of Kamyaran City. Results indicated that 5 purified bacteria contained the LacZ gene. It was also found that although microbial methods are highly sensitive and precise, they are not effective for smaller regions due to their shortage of facilities and equipment.

 

Keywords: LacZ gene, PCR, amplification, coliform

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Entomology and Applied Science Letters is an international double-blind peer reviewed publication which publishes scientific research & review articles related to insects that contain information of interest to a wider audience, e.g. papers bearing on the theoretical, genetic, agricultural, medical and biodiversity issues. Emphasis is also placed on the selection of comprehensive, revisionary or integrated systematics studies of broader biological or zoogeographical relevance. In addition to full-length research articles and reviews, the journal publishes interpretive articles in a Forum section, Short Communications, and Letters to the Editor. The journal publishes reports on all phases of medical entomology and medical acarology, including the systematics and biology of insects, acarines, and other arthropods of public health and veterinary significance.

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Institute of Pharmaceutical Sciences (IPS) , University of Veterinary and Animal Sciences, Lahore Pakistan.
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Entomology and Applied Science Letters supports the submission of entomological papers that contain information of interest to a wider reader groups e. g. papers bearing on taxonomy, phylogeny, biodiversity, ecology, systematic, agriculture, morphology. The selection of comprehensive, revisionary or integrated systematics studies of broader biological or zoogeographical relevance is also important. Distinguished entomologists drawn from different parts of the world serve as honorary members of the Editorial Board. The journal encompasses all the varied aspects of entomological research.