2016 Volume 3 Issue 5

Wound healing potential of various successive fractions obtained from Aegle marmelos (AM) and Mucuna pruriens (MP) in acid burn wound models: An experimental animal study

Fedelic Ashish Toppo, Rajesh Singh Pawar

Traditionally leaves of Aegle marmelos (Linn.) Correa (Rutaceae) and seeds of Mucuna pruriens Linn. (Fabaceae) are applied on cuts and wounds. We report the acid burned wound healing activities of sequential ethyl acetate, methanol and aqueous extracts of AM and MP in acid burn wound models in rats. Forty-eight Wistar-albino rats (220±20g) were divided into eight groups (n=6) and were subjected to acid burn (100 mm2) on the back of their necks. Animals were divided into control group, standard group and treatment groups. The hydrogel was applied topically once daily to the treatment groups. The parameters observed were epithelialization period, wound contraction % and histopathological analysis as indicative of the process of healing. After the 20th day of treatment, wound closure % was 77.75% in control group, for the standard group it was 100%, for the test group III, IV, V, VI, VII and VIII it was 86.21, 94.81, 93.43, 89.66, 94.63 and 80.74%,respectively.Test groups III,IV,VI and VII showed significant (p<0.01)improvement in wound contraction % in comparison to control group. Similarly the period of epithelialization also decreased significantly in test groups. All extracts facilitated the wound healing process as demonstrated by a decrease in the period of epithelialization and faster wound contraction in acid burn wounds. Ethyl acetate and methanolic extract seemed to have the most active component for healing the acid burned wound.


Keywords: Rejuvenate; Bel; Kiwanch; Ameliorated acid burns; Wistar-albino rats


C.P. Sawhney, R. Kaushish, Burns 1989, 15, 132-134.

A.P. Sandford, D.N. Herndon, Chemical burns, In: Herndon DN, editor. Total burn care, 2nd ed., WB Saunders Company, 2002, 475-480.

D.W. Mozingo, A.A. Smith, W.F. McManus, B.A. Pruitt, A.D. Mason, The Journal of Trauma, 1988, 28, 642-647.

R.C. Carlotto, W.J. Peters, P.C. Neligan, L.G. Douglas, J. Beeston, Canadian Journal of Surgery,1996, 39, 205-211.

S.J. Bond, G.C. Schnier, M.J. Sundine, S.P. Maniscalco, D.B. Groff, The Journal of Trauma,1998, 8(44), 523-526.

C. Jelenko, 3rd Chemicals that ‘‘burn’’, The Journal of Trauma, 1974, 14, 65-72.

R.P. Gruber, D.R. Laub, L.M. Vistnes, Plastic and Reconstructive Surgery, 1975, 55, 200-204.

E.C. Davidson, Annals of Surgery, 1927, 85, 481-489.

R. Govindrajan, B. Kumar, M. Vijaykumar, P. Pushpangadan, Journal of Ethnopharmacology, 2007, 114, 103-113.

K.V. George, N. Mohanan, S.S. Nair, Ethnobotanical investigations of Aegle marmelos (Linn.) Corr. In: Ethnobotany and Medicinal Plants of India and Nepal, by V. Singh and A.P. Jain, Scientific Publishers, Jodhpur, 2003: 29-35.

S. Dhankhar, S. Ruhill, M. Balharal, S. Dhankhar, A.K. Chhillar, Journal of Medicinal Plants Research, 2011, 5(9), 1497-1507.

N. Sreeramulu, S. Suthari, A. Ragan, V.S. Raju, Annals of Plant Sciences, 2013, 2(7), 220-229.

V.L. Naidu, A.N. Bahadur, V.K. Kanungo. International Journal of Medicinal and Aromatic Plants, 2014, 4(1), 6-15.

N.P. Yadav, C.S. Chanotia, The Pharma Review2009; 144-150.

C.P. Khare, Indian medicinal plants - An illustrated dictionary. Springer Science + Business Media, LLC, 2007:

M.M. Mohammed, N.A. Ibrahim, F.S. El-Sakhawy, K.M. Mohamed, D.A. Deabes, Natural Product Research,2016, 4, 1-8.

D.S. Kumar, A.K. Muthu, A.A. Smith, R. Manavalan, International Journal of PharmaTech Research, 2010;

(3): 2063-70.

C.P. Khare, Indian medicinal plants - An illustrated dictionary. Springer Science + Business Media, LLC, 2007, 424-425.

M. Rahmatullah, I.J. Mukti, A.K.M.F. Haque, M.A.H. Mollik, K. Parvin, R, Jahan, M.H. Chowdhury, T. Rahman, Advances in Natural and Applied Sciences, 2009, 3(3), 402-418.

A.W. Khan, S. Kotta, S.H. Ansari, R.K. Sharma, A. Kumar, J. Ali, Pharmacognosy Magazine,2013, 9(1): S6–S10.

F.A. Toppo, R.S. Pawar, International Journal of Pharmacy and Pharmaceutical Sciences, 2014; 7(4): 447-452.

S. Kamath, S.G. Rao, K.D. Murthy, K.L. Bairy, S. Bhat, Indian Journal of Experimental Biology, 2006, 44, 902-904.

R.S. Pawar, P.K. Chaurasiya, H. Rajak, P.K. Singour, F.A. Toppo, A. Jain, Indian Journal of Pharmacology, 2013, 45(5), 474-478.

H. Tsuchiya, M. Sato, T. Miyazaki, S. Fujiwara, S. Tanigaki, M. Ohyama, T. Tanaka, Journal of Ethnopharmacology, 1996, 50, 27-34.

D.A. Lewis, P.J. Hanson, Progress in Medicinal Chemistry, 1991, 28, 210-229.

O, Senel, O.G. Cetinkale, F. Ahcioglu, R. Bulan, Annals of Plastic Surgery, 1997, 39, 516.

K. Kiran, M. Asad, Indian Journal of Experimental Biology, 2008, 46, 777.

S. Szabo, S. Kusstatscher, G. Sakoulas, Z. Sandor, A. Vincze, M. Jadus,Scandinavian Journal of Gastroenterology,1995, 15-8.

P. Buntrock, K.D. Jentzsch, G. Heder, Experimental Pathology, 1982, 21, 62-67.

A. Ulubelen, G. Topcu, N. Tan, S. Olcal, C. Johansson, M. Ucer, H. Birman, S. Tamer, Journal of Ethnopharmacology,1995, 45, 193-197.

A.A. Hemmati, F. Mohammadian, Journal of Herbs Spices and Medicinal plants, 2000, 7, 41-46.

S.W. Choi, B.W. Son, Y.S. Son, Y.I. Prak, S.K. Lee, M.H. Chung, British Journal of Dermatology, 2001, 145, 535-545.

K.L. Bairy, Journal of Natural Remedies, 2002, 2, 11-20.

K. Annan, P.J. Houghton, Journal of Ethnopharmacology, 2007, 119, 141-144.

H.S. Muhammad, S. Muhammad, African Journal of Biotechnology, 2005, 4, 934-937.

S.R. Gregory, M.T. Piccolo, M.S. Piccolo, J.P. Heggers, Journal of Alternative and Complementary Medicine, 2002, 8, 77-83.

B.M. Rodriguez, N.I. Cruz, A. Suarez, Plastic and Reconstructive Surgery, 1988, 81, 386-389.

A. Shukla, A.M. Rasik, B.N. Dhawan, Phytotherapy Research, 1999, 13, 50-54.

P. Rani, N. Khullar. Phytotherapy Research, 2004, 8(8), 670-3.

M. Rajadurai, P.S. Prince,Singapore Medical Journal, 2005, 46(2), 78-81.

Y.B. Tripathi, A.K. Upadhyay, Current Science, 2001, 80(11), 1377-1378.

Y. Rajeshwar, M. Gupta, U.K. Mazumder, Iranian Journal of Pharmacology and Therapeutics, 2005, 4(1), 32-35.

K.S. Purna, P.R. Neelakanta, M. Babu. Indian Journal of Experimental Biology, 1995, 33, 673-676.

B.K. Manjunatha, S.M. Vidhya, V. Krishna, K.L. Mankani, Indian Journal of Pharmaceutical Sciences, 2006, 68, 380-4.

D.K. Sekar, G. Kumar, L. Karthik, K.V.B. Rao. Asian Journal of Plant Science and Research, 2011, 1, 8-17.

G. Griebel, G. Perrault, S. Tan, H. Schoemaker, D.J. Sanger, Neuropharmacology, 1999, 38, 965-977.

J.H. Medina, H. Viola, C. Wolfman, M. Marder, C. Wasowski, D. Calvo, A.C. Paladidni, Phytomedicine,1997, 5, 235-243.

B. Mamtha, K. Kavitha, K.K. Srinivasan, P.G. Shivananda, Indian Journal of Pharmacology, 2004, 36, 41.

N.E. Hemandez, M.L. Tereschuk, L.R. Abdala, Journal of Ethnopharmacology, 2000, 73, 317-22.

T. Ojala, S. Remes, P. Haansuu, H. Vuorela, R. Hiltunen, K, Haahtela, P. Vuorela, Journal of Ethnopharmacology, 2000; 73: 299-305.

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