Creative Commons License 2016 Volume 3 Issue 2

Secondary metabolites isolated from the bacterial complex of Apismellifera and their action against Atta mexicana


Rivera A, Romero O, Hernandez F, Badillo F, Aragon A
Abstract

Damage caused by insects in agriculture has led to a significant decrease in crop quality, yield and economic losses. So this situation has led to the indiscriminate use of synthetic chemicals that have sought to mitigate negative impacts on the crops, but without considering the pressure and imbalances that are causing on ecosystems. Therefore it is necessary to direct research into new biotechnological alternatives, focused on the control of pests which lead to the decrease of the acquisition of external inputs in agroecosystems, framing production systems in sustainable agriculture that produces healthy, sufficient food and quality cost-effective, socially acceptable and without harming the environment. We evaluated the ability of metabolites produced by bacterias isolated from Apis mellifera as insecticides against Atta mexicana. Metabolites extraction was carrying out from the Enterobactericiae isolated, Klebsiella, Pseudomonas, Proteus and Yersinia, by using an adsorbent resin and elution with 99% ethanol. The insecticidal activity of metabolites was tested in vitro using experimental units against Atta mexicana. Yersinia metabolites showed insecticidal activity over Atta mexicana showing an accumulative mortality of 92.5%. Protein profile was performed on the metabolites, using dodecyl sulphate-polyacrylamide gel, where variability was observed in weight and number of protein bands, predominantly 70 kDa. Yersinia showed protein bands of 10 and 70 kDa, the weight could be comparable to Cry endotoxins of Bacillus thuringensis. Results suggest that Atta mexicana can be considered as a promissory source of by bacterias with varying activity that could be used as alternatives biotechnological protection crops against Atta mexicana.

 

Key words: Bacterial complex, secondary metabolites, insecticidal activity, Apismellifera, Atta Mexicana.


Share:
References

F. Hadacek,H.Greger,Phytochemical Analysis Journal, 2000, 11, 137-147

M.V. Bertorelli,J. Montilla J, .J. Hernandez J, Revista de la Facultad de Agronomía, 2006, 23, 310-318

S. Thomashow,M. Weller,Plant-Microbe Interactions, 1996, 1,187-235

S. García, T. Romer,P. Orduz,ActualidadesBiológicas, 2012, 34, 5-19

E. Pushpalatha,J.Muthukrishnan,Journal of Applied Entomology, 1999,123, 369-373

C.H. Liu,W.X. Zou,H. Lu,R.X. Tan,Journal of Biotechnology, 2001, 88,277-282

R. Tasao, E. Romanchuk, J. Peterson,R. Coats,BioMed Central Ecology, 2002, 2,1-8

S. Nathan,Bioresource Technology, 2006,98, 1856-1860

Omura S. The search for bioactive compounds from microorganisms: Fungicides and antibacterial agents.

Springer Seriesin Contemporary Bioscience, 1992, pp. 213-243

V. Martinson, B. Danforth B, R. Minckley,O. Rueppell, O. Tingek,N. Moran,Molecular Ecology,2011,20, 619-628

R.R. Colwell, Biotechnology Advances,2002, 20,215-228

L.V. Manzi,J.C.Mayz, Revista de la Sociedad Venezolana de Microbiología, 2003, 23, 85-88

J.E. Casida, G.B. Quistad,Annual Review of Entomology, 1998,43,1-16

Metcalf C.L., Flint W.P. Insectos destructivos e insectos útiles. México, CECSA, 1976, pp. 1208.

J.M. Maes,W.P.Mackay, Revista Nicaragüense de Entomología,1993, 23, 1-46

Della L. (2003). Hormigas de importancia económica en la región Neotropical: Introducción a las hormigas de la Región Neotropical.Bógota, Colombia; Instituto Humboldt, 2003, pp.337-349

Fowler H., Bernardi J., Delabie C., Forti L.C., Pereira-Da-Silva V. Major ant problems of South America: Applied Myrmecology; a world perspective.Boulder Colorado, Westview Press, USA, 1990, pp. 3-14

Escobar D., García C., Rentería N.Y., Neita M. Manejo y control de hormiga arriera (Attasp&Acromyrmexsp) en

sistemas de producción de importancia económica en el departamento del Chocó. Cartilla 1 y 2. Ministerio de

Agricultura-PRONATTA-Universidad Tecnológica del Chocó. CO, 2002, pp. 53.

G. Sierra, T. Romero,P.Orduz,Actualidades Biológicas, 2012, 34, 5-19.

Gilliam M. Microbial Ecology of honeybees. Proc XXXIth International Apicultural Congr. Warsaw. ApimondiaPubl House, Bucharest, 1987, pp. 217-220

M. Gilliam, B.J. Lorenz,G.V. Richardson,Microbios, 1988,55, 95-114

M. Gilliam,S. Taber,Journal of Invertertebrate Pathology, 1991, 58, 286-289

P. White,Journal of Pathology Bacteriology, 1921, 24: 64-72

V. Martinson, J. Moy,N. Morán,AppliedEnvironmentMicrobiology, 2012,78, 2830-2840

R. Dillon,V. Dillon,Annual Review of Entomology, 2004,49, 71-92

A. Klein A, B. Vaissiere, J. Cane, I. Steffan-Dewenter, S. Cunningham,C. Kremen,Containing Papers of a Biological Character, 2007,274,303-313

M.A. Aizen, L.A. Garibaldi, S.A. Cunningham,A.M. Klein,CurrentBiology, 2008,1, 1572-1575

P. Neumann,N.Carreck,Journal of Apicultural Research, 2010, 49, 1-6

D. vanEngelsdorp D, D. Caron D, J. Hayes, R. Underwood R, M. Henson, K. Rennich, A. Spleen A, M. Andree,

R. Snyder, K. Lee, K. Roccasecca, M. Wilson, J. Wilkes, E. Lengerich,J. Pettis,Journal of Apicultural Research,

, 51,115-124

M. Spleen M, E. Lengerich, K. Renich K, D. Caron D, R. Ros,S. Pettis S, M. Henson, T. Wilkes, M. Wilson M, J. Stitzinger, K. Lee K, M. Andree, R. Synder R, D.vanEngelsdorp, Journal of Apicultural Research, 2013,52, 44-53

R. Heather, D. Rios, V. Walker-Sperling, G. Roeselers,I. Newton, Plos One, 2012,7, 1-11

H.V. Corby, P. Maes,K.E.Anderson, Plos One, 2014, 9, 1-13

Schmid-Hempel P. Parasites in social insects. Princeton University Press, Princeton, NJ, 1998, pp. 392

J. Parkhill J, B.W. Wren, N.R. Thomson,R.W. Titball, M.T. Holden, M.B. Prentice, M. Sebaihia, K.D. James, C. Churcher C, K.L. Mungall, S. Baker, D. Basham, S.D. Bentley, K. Brooks, A.M. Cerdeño-Tárraga, T. Chillingworth, A. Cronin, R,M. Davies, P. Davis, G. Dougan, T. Feltwell, N. Hamlin, S. Holroyd S, K. Jagels, A.V.

Karlyshev, S. Leather, S. Moule, P.C. Oyston, M. Quail, K. Rutherford, M. Simmonds, J. Skelton, K. Stevens K, S.Whitehead,B.G.Barrell,Nature, 2001, 413,523-527

J.M. Landsberg,A.S. Jones, R. Rothnagel R, N.J. Busby,S.D.Marshall,M.R. Simpson, J.S. Lott, B. Hankamer,M.R.Hurst,(2011). 3D structure of the Yersinia entomophaga toxin complex and implications for insecticidal activity. Proceedings of the National Academy of Sciences of the United States of America, 2011,108, 20544-20549

R.H. Hurst,S.A. Jones, T. Binglin T,L.A. Harper,T.A. Jackson,T.R. Glare,Journal of Bacteriology, 2011,193, 1966-1980

R.H. Ffrench-Constant,A. Dowling,N.R.Waterfield,Toxicon Journal, 2007,49, 436-451

M.B. Blackburn, P.A.W. Martin,D. Kuhar, R.R. Farrar,D.E. GundersenRindal,PLoSOne, 2011,6, 1-7

B.E. Tabashnik, N. Finson, M.W. Johnson,W.J. Moar, Applied and Environmental Microbiology, 1993,59,1332–1335

B. D. Plikaytis, G.M. Carlone,B.B.Plikaytis, Journal of General Microbiology, 1986,132,2653-2660

P. Wirth,A. Romano, Journal of Chromatogaphy,1995,698, 123-143

T.H. Steinberg, Methods in Enzymology, 2009,463, 541-563

C. Del Rincón, J. Méndez,J. Ibarra,Folia Entomológica Mexicana, 2006,45,157-164

R. Iñigo, A. Estela A, M. Porcar, C. Martínez C, J. Oguiza, J. Ferré,P. Caballero, Applied Environmental Microbiology, 2006,72, 4796-4804

N. Crickmore, D.R. Zeigler, E. Schnepf, J. Van Rie, D. Lereclus, J. Baum, A. Bravo,D.H. Dean,Microbiology and Molecular BiologyReviews, 1998,62, 807-813

King L., Wong G, Rodríguez C, Luna-Olvera H.Avances recientes en la biotecnología en Bacillusthuringensis.

Universidad de Nuevo León, 1996, pp. 64-68

E. Shneff, N. Crickmore N, R. Van, D. Lereclus, J. Baum, J. Feitelson, D. Zeigler D.H. Dean,Microbiology Molecular Biology Review, 1998,62, 775-806

CrickmoreN. The diversity of Bacillus thuringensis delta-endotoxins: Entonopathogenic bacteria: from laboratory to field application.Kluwer Academic Publisher, Dordrecht, 2000, pp. 41-46

M. Soberon,A. Bravo,Biotecnologia, 2007,14, 303-314

C. González, G. Farino´s, S. Caccia, M. Díaz, P. Castañera, G. Leonardi, B. Giordana,J. Ferre,Applied and Environmental Microbiology, 2006,72, 2594-2600


Entomology and Applied Science Letters is an international double-blind peer reviewed publication which publishes scientific research & review articles related to insects that contain information of interest to a wider audience, e.g. papers bearing on the theoretical, genetic, agricultural, medical and biodiversity issues. Emphasis is also placed on the selection of comprehensive, revisionary or integrated systematics studies of broader biological or zoogeographical relevance. In addition to full-length research articles and reviews, the journal publishes interpretive articles in a Forum section, Short Communications, and Letters to the Editor. The journal publishes reports on all phases of medical entomology and medical acarology, including the systematics and biology of insects, acarines, and other arthropods of public health and veterinary significance.

Announcement and Advertisement
Announcements regarding scientific activities such as conferences, symposium, are published for free. Advertisements can be either published or placed on website as banners.

Publisher
Institute of Pharmaceutical Sciences (IPS) , University of Veterinary and Animal Sciences, Lahore Pakistan.
open access
Associations
Entomology and Applied Science Letters supports the submission of entomological papers that contain information of interest to a wider reader groups e. g. papers bearing on taxonomy, phylogeny, biodiversity, ecology, systematic, agriculture, morphology. The selection of comprehensive, revisionary or integrated systematics studies of broader biological or zoogeographical relevance is also important. Distinguished entomologists drawn from different parts of the world serve as honorary members of the Editorial Board. The journal encompasses all the varied aspects of entomological research.