This investigation aims to assess the chemical composition and antidermatophytic properties of Nectaroscordum tripedale against some pathogenic dermatophyte strains. In vitro antidermatophytic effects of N. tripedale extract on Trichophyton mentagrophytes, Microsporum canis, and Microsporum gypseum was determined using the determination of minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) based on the broth microdilution method, according to the protocol M38-A2 of the Clinical and Laboratory Standards Institute (CLSI) for filamentous fungi with some modifications. The components of the N. tripedale extract were identified by gas chromatography/mass spectroscopy (GC/MS) analysis. The results demonstrated that N. tripedale extract had both fungistatic and fungicidal activities with the MIC and MFC ranging from 16.6 to 66 mg/ml. The main compounds are decadienal (11.1%), hexadecanoic acid (10.3%), and heptadecane (9.5%), respectively. Obtained results of this investigation recommend a first step in the search of new antidermatophytic agent and support the use of N. tripedale in the folk medicine for dermatophytic infections.
Kauffman CA. Fungal infections. Proc Am Thorac Soc 2006; 3: 35–40.
Mahmoudvand H, Sepahvand A, Jahanbakhsh S, Ezatpour B, Ayatollahi Mousavi SA. Evaluation of antifungal activities of the essential oil and various extracts of Nigella sativa and its main component, thymoquinone against pathogenic dermatophyte strains. J Mycol Med. 2014a; 24(4):155-61.
Sepahvand A, Abdi A, Shirkhani Y, Fallahi SH, Tarrahi M, Soleimannejad S. Dermatophytosis in Western part of Iran, Khorramabad. Asia journal of Biological science 2009; 2(3): 58-65.
De Pauw BE. Is there a need for new antifungal agents? Clin Microbiol Infect 2000; 6, 23–28.
Watanabe S. Present state and future direction of topical antifungals. Japaneses J Med Mycol 1999; 40: 151–155.
Mahmoudvand H, Sharififar F, RahmatMS, Tavakoli R, Dezaki ES, Jahanbakhsh S, Sharifi I. Evaluation of antileishmanial activity and cytotoxicity of the extracts of Berberis vulgaris and Nigella sativa against Leishmaniatropica. J Vector Borne Dis. 2014b: 51(4): 294-9.
Mahmoudvand H, Asadi A, Harandi MF, Sharififar F, Jahanbakhsh S, Dezaki ES. In vitro lethal effects of various extracts of Nigella sativa seed on hydatid cyst protoscoleces.Iran J Basic Med Sci. 2014c: 17(12): 1001-6.
Rocha LG, Almeida JR, Macedo RO, Barbosa-Filho JM. A review of natural products with antileishmanial activity. Phytomedicin 2005; 12: 514-535.
Mozaffarian V. Trees and Shrubs of Iran, Farhang Moaser, Tehran, Iran, 1st edition, 2005.
Shafie-Zadeh F. Lorestan medicinal plants. Lorestan University of Medical Sciences. Tehran: Hayyan press; 2002.
Ezatpour B, Azami M, Motamedi M, Rashidipour M, Mahmoudvand H, Alirezaei M, et al. Chemical Composition, in Vitro Antibacterial and Cytotoxicity Effect of Nectaroscordum tripedale Extract. Herb. Med. J. 2016;1(1):29-36.
Mahmodvand H, Torkashvand M, Kheirandish F, Ezatpour B, Mirbadie SR, Jahanbakhsh S. Assessment of the In Vitro antileishmanial and cytotoxic activity of Nectaroscordum tripedale fruit extract. Entomology and Applied Science Letters, 2016, 3, 3S:1-5
Mahmoudvand H, Ezatpour B, Rashidipour M, Mirbadie SR, Mahmoudvand H. Report: Evaluation of the scolicidal effects of Nectaroscordum tripedale extract and its acute toxicity in mice model. Pak J Pharm Sci. 2016 Nov;29(6):2125-2128.
Jahanbakhsh S, Azadpour M, Tavakoli Kareshk A, Keyhani A. Mahmoudvand H. Zataria multiflora Bioss: lethal effects of methanolic extract against protoscoleces of Echinococcus granulosus. J Parasit Dis 2015,DOI 10.1007/s12639-015-0670-4.
Mahmoudvand H, Sepahvand P, Jahanbakhsh S, Azadpour M. Evaluation of the antileishmanial and cytotoxic effects of various extracts of garlic (Allium sativum) on Leishmania tropica. J Parasit Dis. 2016b; 40(2):423-6.
Ghiasvand AR, Nasseri M, Farsizaeh S, Meshkatalsadat MH, Sadeghi-Sarabi R, Shadabi S, et al. Chemical characterization of cultivated Tagetes minuta L. by use of ultrasound-assisted head space SPME and GC–MS. Chromatographia. 2011;73:1031-5.
CLSI/Clinical and Laboratory Standards Institute – (2002
. Reference method for broth dilution antifungal susceptibility testing of conidium-forming filamentous fungi. Wayne. (Approved Standard M38-A).
Mahmoudvand H, Kheirandish F, Dezaki ES, Shamsaddini S, Harandi MF, Chemical composition, efficacy and safety of Pistacia vera (var. Fandoghi) to inactivate protoscoleces during hydatid cyst surgery. Biomedicine & Pharmacotherapy, 2016c: 82: 393–398.
McCutcheon AR, Ellis SM, Hancock REW, Tower GNH. Antibiotic screening of medicinal plants of the British Columbian native peoples. J Ethnopharmacol 1992; 37: 213– 223.
Kheirandish F, Delfan B, Mahmoudvand H, Moradi N, Ezatpour B, Ebrahimzadeh F, Rashidipour M. Antileishmanial, antioxidant, and cytotoxic activities of Quercus infectoria Olivier extract. Biomed Pharmacother. 2016; 82:208-15.
Saedi Dezaki E, Mahmoudvand H, Sharififar F, Fallahi S, Monzote L, Ezatkhah F. (2015). Chemical composition along with anti-leishmanial and cytotoxic activity of Zataria multiflora. Pharm Biol. 2015: 8: 1-7.
Ncube NS, Afolayan AJ, Okoh AI. Assessment techniques of antimicrobial properties of natural compounds of plant origin: current methods and future trends. Africa J Biotech 2008; 7: 1797–1806.
Yesil-Celiktas O, Nartop P, Gurel A, Bedir E, Vardar-Sukan F. Determination of phenolic content and antioxidant activity of extracts obtained from Rosmarinus officinalis' calli. J Plant Physiol. 2007 Nov;164(11):1536-42.
Mahmoudvand H, Tavakoli Oliaei R, Mirbadie SR, Kheirandish F, Tavakoli Kareshk A, Ezatpour B,Mahmoudvand H. Efficacy and Safety of Bunium Persicum (Boiss) to Inactivate Protoscoleces during Hydatid Cyst Operations. Surg Infect (Larchmt). 2016d Aug 8. [Epub ahead of print].
Mahmoudvand H, Saedi Dezaki E, Ezatpour B, Sharifi I, Kheirandish F, Rashidipour M. In vitro and In vivo antileishmanial activities of Pistacia vera essential Oil. Planta Medica 2016f: 82(4): 279-284.
Gołębiowski M, Cerkowniak M, Urbanek A, Dawgul M, Kamysz W, Boguś MI, Sosnowska D, Stepnowski P. Antimicrobial activity of untypical lipid compounds in the cuticular and internal lipids of four fly species. J Appl Microbiol. 2014; 116(2):269-87.
Trombetta D, Saija A, Bisignano G, Arena S, Caruso S, Mazzanti G, Uccella N, Castelli F. Study on the mechanisms of the antibacterial action of some plant alpha,beta-unsaturated aldehydes. Lett Appl Microbiol. 2002; 35(4):285-90.
Du YH, Li JL, Jia RY, Yin ZQ, Li XT, Lv C, Ye G, Zhang L, Zhang YQ.Acaricidal activity of four fractions and octadecanoic acid-tetrahydrofuran-3,4-diyl ester isolated from chloroform extracts of neem (Azadirachta indica) oil against Sarcoptes scabiei var. cuniculi larvae in vitro. Vet Parasitol. 2009 7;163(1-2):175-8.