Effects of Graded Levels of Tannin-Containing Pomegranate Peel (Punica granatum) by Irradiation on In Vitro Methane Production and Population of Protozoa

This study was conducted to determine the effect of pomegranate peel tannin on in vitro CH4 production and
protozoa population. Electron beam and gamma ray irradiation exposed to pomegranate peel at doses of 5, 10, 15 and 20 kGy to evaluate condensed tannin (CT). Three ruminally fistulated rams used to obtain ruminal fluid for in vitro CH4 production and protozoa population. Data were analyzed by using the GLM procedure. The results showed that both gamma and electron beam irradiation significantly decreased condensed tannin than control. There was no difference between gamma and electron beam irradiation concerning condensed tannin reduction. Irradiation did not change methane production. Irradiation had no significant effect on total population and five genuses of protozoa (Diplodinium, Entodinium, Dasytricha, Isotricha and Ophryoscolex). Although, there was no correlation between condensed tannin and CH4 production, a significant negative correlation coefficients between methane production and total protozoa population and between gas volume at 24 h and total protozoa population of gamma irradiated pomegranate peel were observed (P<0.05). In conclusion, the results showed that degrading and reducing of condensed tannin polymer of pomegranate peel by irradiation was not essentially related to the
methanogenesis and protozoa population.

Keywords: Condensed tannin, Irradiation, Methane, Protozoa.

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