Isolation and identification of bacterial contamination by phenotypic and genotypic methods in Artemia cysts and assessment of antibiotic susceptibility pattern of Vibrio species isolated in Artemia Research Center, Urmia

Shrimp farming is a sufficiently large and mature industry to have an effective range of antimicrobial agents for most bacterial diseases in shrimp culture. The objectives of this study were to investigate the occurrence of bacterial contamination by conventional and molecular techniques and assessment of antibacterial susceptibility of four antibiotic agents’ ciprofloxacin, erythromycin, tetracycline and streptomycin by disc diffusion applied on them. Of 40 Brine shrimp (Artemia urmiana) samples analyzed 42.5% was recorded positive for vibrio Spp and 11%, 16.5%, 17%, 9%, 4% were positive for Pseudomonas aeruginosa, coccus spp, E. coli, Bacillus cereus, Salmonella typhimurium respectively. Of 17 (42.5%) vibrio spp 7 (41.17%), 4 (23.52%) and 6 (35.29%) identified as V. parahaemolyticus, V. Vulnificus and V. harveyi respectively. Consequently of 17 isolates of vibrio spp 8 of them were resistant to all used antibiotic agents separately.

Keywords: Bacterial contamination, Vibrio species, Artemia cysts, Antibiotic susceptibility

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