Determination of metabolic profiles at pre partum and postpartum stages in fresian x bunaji and bunaji cows

A study of the metabolic profile reflecting metabolic energy status prior to artificial insemination, during the different trimesters of pregnancy and at postpartum period (PP), in Friesian x Bunaji (FR x BJ) and Bunaji (BJ) cows, placed on the same feeding regimen was conducted. Twenty eight cows comprising of 11 FR x BJ and 17 BJ were observed for normal cyclicity. Only 8 cows (4 FR x BJ and 4 BJ) that showed normal cyclicity were used for the study. They where synchronized using a single intramuscular injection of 25 mg of PGF2α and inseminated using Friesian semen. Blood samples were obtained every morning before feeding during pre–insemination, pregnancy and PP at 30 days interval. The samples obtained were processed for serum and assayed for metabolites and electrolytes. The pre–insemination (control) values (range) for metabolic parameters for both breeds were glucose
(GL) (2.17 ± 0.44 – 2.29 ± 0.32 mMol/L), triglycerides (TRG) (0.61 ± 0.07 – 0.74 ± 0.08 mMol/L), cholesterol (CHL) (4.85 ± 0.77 – 5.15 ± 0.58 mMol/L), urea (UR) (7.41 ± 0.97 – 10.46 ± 2.68 mMol/L), creatinine (CRT) (188.68 ± 15.32 – 135.13 ± 23.05 mMol/L), sodium (Na+) (152.38 ± 7.43 – 152.75 ± 5.53 mMol/L), potassium (K+) (4.30 ± 0.29 – 4.31 ± 0.29 mMol/L) chloride (Cl–) (103.38 ± 5.16 – 106.25 ± 3.69 mMol/L), bicarbonate (HCO3 2–) (17.23 ± 2.72 – 23.00 ± 2.49 mMol/L), total protein (TP) (55.19 ± 5.16 – 59.54 ± 6.05 g/l) and albumin (ALB) (27.69 ± 3.10 – 29.02 ± 5.27 g/l). There was increase in GL, TRG, CHL, HCO3 2–, Ca2+, TP, and ALB levels in the two breeds, with onset of pregnancy when compared to the control, but CRT, UR, Na+, K+, and Cl– levels decreased with onset of pregnancy. There were no significant differences (P > 0.05) in the levels of GL, UR, CHL, Na+, K+, HCO3\ 2–, Ca2+, TP and ALB between the two breeds, throughout the reproductive stages. However, significant differences were obtained in other metabolic parameters between the breeds at different reproductive stages. This research has provided a baseline data on metabolic parameters of FR x BJ and BJ cows prior to insemination, during the different trimesters of pregnancy and at postpartum period, which are considered valuable in future clinical and experimental applications.

Keywords: Bunaji, Fresian x Bunaji, metabolic profile.

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