Creative Commons License 2018 Volume 5 Issue 1

Evaluation of Protoscolicidal Effects of Nectaroscordum Koelzi Methanolic Extract Against Hydatid Cyst Protoscoleces

Nasrin Galehdar, Massumeh Niazi, Sareh Jahanbakhsh, Hormoz Mahmoudvand, Abdolreza Rouientan

Here, we studied in vitro scolicidal effects of Nectaroscordum koelzi (N. koelzi) extract against hydatid cysts protoscoleces. Collected protoscoleces from sheep livers with fertile hydatid cysts were exposed with different concentrations of the extract (62.5-500 mg/mL) for 10-60 min in vitro. Then viability of protoscoleces was evaluated using the eosin exclusion test (0.1% eosin staining). Findings revealed that N. koelzi extract, at the concentrations of 250 and 500 mg/mL completely killed protoscoleces after 10, 20 min of exposure, respectively. The results also indicated that the survival rate of protoscoleces in the negative and positive controls was 95.4% and 0% after 60 and 10 min of exposure, respectively; indicating that N. koelzi extract at all of these concentrations had significantly higher (P < 0.05) protoscolicidal activity than the control group. The findings indicated potential of N. koelzi methanolic extract as a natural source for the producing of new scolicidal agent for use in hydatid cyst surgery.


Kasper DL, Fauci AS, Longo DL, Anthony S, Stephen L, Jameson JL. Harrison's principles of internal medicine.16thed. New York.McGraw_Hill Co. 2005; pp: 1275.

Eckert J, Deplazes P. Biological, epidemiolog-ical, and clinical aspects of echinococcosis, a zoonosis of increasing concern. Clin Microbi-ol Rev 2004 17: 107–35.

Brunetti E, Kern P, Vuitton DA. Writing Panel for the WHO-IWGE Expert consensus for the diagnosis and treatment of cystic and alveolar echinococcosis in humans. Acta Trop 2010; 114(1): 1-16.

Rokni MB. Echinococcosis/hydatidosis in Iran. Iranian J Parasitol. 2009 May; 4(2): 1-16.

Arti H, Yousofi Darani H. Fibular hydatid cyst. Indian J Orthop. 2007 July-Sept; 41(3): 244-5.

Jahani MR, Roohollahi GH, Gharavi MJ. Splen-ic Hydatid cyst in a 20 years old solidier. Mil Med 2004 Apr; 169: 77-80.

Brunicardi F, Dana k, Thmothy R. Schwartz's Principle of Surgery, 8th ed. New York, The McGraw-Hill Companies; 2006. 1163-1165.

Kliegma R, Jenson H, Behrman R, Stanton B. Nelson’s Textbook of Pediatrics.17th ed. Phil-adelphia: Saunders, 2004: 1448-1458.

McManus DP, Zhang W, Li J. Echinococcosis. Lancet 2003; 362: 1295–304.

Junghanss T, da Silva AM, Horton J, et al. Clin-ical management of cystic echinococcosis: state of the art, problems, and perspectives. Am J Trop Med Hyg 2008; 79(3): 301-311.

Mahmoudvand H, Fasihi Harandi M, Shakibaie M, et al. Scolicidal effects of biogen-ic selenium nanoparticles against protoscoli-ces of hydatid cysts. Int J Surg 2014;12 (5): 399-403.

Khaldi M, Mohammad S, Kallel J, Khouja N. Brain hydatidosis: Report on 117 cases. Childs Nerv Syst. 2000 Nov; 16(10-11): 65-769.

Rajabi MA. Fatal reactions and methaemoglo-binaemia after silver nitrate irrigation of hy-datid cyst. Surg Pract 2009; 13: 2–7.

Sahin M, Eryilmaz R, Bulbuloglu E. The effect of scolicidal agents on liver and biliary tree (experimental study). J Invest Surg 2004; 17(6):323-6.

Cosa P, Vlietinck AJ, Berghe DV, Mae L. Anti-infective potential of natural products: How to develop a stronger in vitro ‘proof-of-concept’. J Ethnopharmacol 2006; 106: 290-302.

Mozaffarian V. Trees and Shrubs of Iran, Far-hang Moaser, Tehran, Iran, 1st edition, 2005.

Shafie-Zadeh F. Lorestan medicinal plants. Lorestan University of Medical Sciences. Teh-ran: Hayyan press; 2002.

Jahanbakhsh S, Azadpour M, Tavakoli Ka-reshk A, Keyhani A. Mahmoudvand H. Zataria multiflora Bioss: lethal effects of methanolic extract against protoscoleces of Echinococcus granulosus. J Parasit Dis 2015, DOI 10.1007/s12639-015-0670-4.

Mahmoudvand H, Sepahvand P, Jahanbakhsh S, Azadpour M. Evaluation of the antileish-manial and cytotoxic effects of various ex-tracts of garlic (Allium sativum) on Leishma-nia tropica. J Parasit Dis. 2016b; 40(2):423-6.

Mahmoudvand H, Sharififar F, Saedi Dezaki E, Ezatpour B, Jahanbakhsh S, Fasihi Harand M (2014c) Protoscolecidal effect of Berberis vulgaris root extract and its main compound, berberine in cystic echinococcosis. Iran J Par-asitol 9(4):26–34.

Yousofi Darani H, Avijgan M, Karimi K, Manouchehri K, Masood J. Seroepidemiology of hydatid cyst in Chaharmahal and Bakhtiari province. Iranian J Public Health. 2003; 23(2): 31-3.

Feyzi E, Bagheri A, Matin S. Splenic Hydatid Cyst: A Case Report. J Ardabil Univ Med Sci. 2011; 11(4): 390-394.

Mahmoudvand H1, Asadi A2, Harandi MF3, Sharififar F4, Jahanbakhsh S1, Dezaki ES3. In vitro lethal effects of various extracts of Ni-gella sativa seed on hydatid cyst protoscole-ces. Iran J Basic Med Sci. 2014 Dec;17(12):1001-6.

Mahmoudvand H1, Kheirandish F1, Dezaki ES2, Shamsaddini S2, Harandi MF3. Biomed Pharmacother. Chemical composition, effica-cy and safety of Pistacia vera (var. Fandoghi) to inactivate protoscoleces during hydatid cyst surgery. 2016 Aug; 82:393-8. doi: 10.1016/j.biopha.2016.05.012. Epub 2016 May 31.

Mahmoudvand H1, Mirbadie SR2, Ghasemi Kia M3, Badparva E1, Shamsadini Lori S4, Fasihi Harandi M4. Efficacy of Pistacia khin-juk Fruits on Viability of Hydatid Cyst Proto-scoleces and Its Acute Toxicity in Mice Mod-el. Iran J Parasitol. 2016 Jul-Sep;11(3):383-388.

Mahmoudvand H1, Kheirandish F2, Ghasemi Kia M3, Tavakoli Kareshk A4, Yarahmadi M4. Nat Prod Res.Chemical composition, proto-scolicidal effects and acute toxicity of Pistacia atlantica Desf. fruit extract. 2016;30(10): 1208-11.doi: 10.1080/14786419.2015.1046868.Epub 2015Aug 7.

Mahmoudvand H1, Tavakoli Oliaei R2, Mir-badie SR3, Kheirandish F1, Tavakoli Kareshk A2, Ezatpour B1, Mahmoudvand H4. Efficacy and Safety of Bunium Persicum (Boiss) to In-activate Protoscoleces during Hydatid Cyst Operations. Surg Infect (Larchmt). 2016 Dec;17(6):713-719. Epub 2016 Aug 8.

Mahmoudvand H1, Fallahi S2, Mahmoudvand H3, Shakibaie M4, Harandi MF5, Dezaki Effi-cacy of Myrtus communis L. to Inactivate the Hydatid Cyst Protoscoleces.ES6J Invest Surg. 2016 Jun;29(3):137-43. doi: 10.3109/08941939.2015.1088601. Epub 2015 Dec 18.

Ezatpour B, Azami M, Motamedi M, Ra-shidipour M, Mahmoudvand H, Alirezaei M, et al. Chemical Composition, in Vitro Antibac-terial and Cytotoxicity Effect of Nectaroscor-dum tripedale Extract. Herb. Med. J. 2016;1(1):29-36.

Mahmodvand H, Torkashvand M, Kheiran-dish F, Ezatpour B, Mirbadie SR, Jahanbakhsh S. Assessment of the in Vitro antileishmanial and cytotoxic activity of Nectaroscordum tripedale fruit extract. Entomology and Ap-plied Science Letters, 2016, 3, 3S:1-5.

Mahmoudvand H, Ezatpour B, Rashidipour M, Mirbadie SR, Mahmoudvand H. Report: Eval-uation of the scolicidal effects of Nec-taroscordum tripedale extract and its acute toxicity in mice model. Pak J Pharm Sci. 2016 Nov;29(6):2125-2128.

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Institute of Pharmaceutical Sciences (IPS) , University of Veterinary and Animal Sciences, Lahore Pakistan.
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Entomology and Applied Science Letters supports the submission of entomological papers that contain information of interest to a wider reader groups e. g. papers bearing on taxonomy, phylogeny, biodiversity, ecology, systematic, agriculture, morphology. The selection of comprehensive, revisionary or integrated systematics studies of broader biological or zoogeographical relevance is also important. Distinguished entomologists drawn from different parts of the world serve as honorary members of the Editorial Board. The journal encompasses all the varied aspects of entomological research.