Comparison of the Effect of Two Groups of Resin Cements (Panavia F2 and Rely X Plus) and Zinc Phosphate Cement (Harvard) On the Induc-tion of IL-6 by Category L-929 Mouse Fibroblast

Introduction: Resin cement, despite its biocompatibility, has been extensively applied in restorative dentistry during recent years. The resin matrix comprises one or more ‘light’ co-monomer systems (e.g. HEMA) and ‘heavy’ monomer systems (including Bis-GMA) to decrease the monomers’ viscosity and to increase the bonding strength to dentine. Acrylates, mainly methacrylates, have been revealed to cause cytotoxic impacts. Objective: This investigation contrasted the impact of resin cement (Rely X Plus and Panavia F2) and zinc phosphate cement (Harvard) on the initiation of IL-6 by category L-929 mouse fibroblast. Method: One resin cement (Panavia F2), one resin ionomer cement (Rely X Plus) and Harvard cement (Zinc Phosphate) were tested. The cement was prepared in hollow glass tubes (inner diameter of 5 mm, the height of 2mm) and 10 samples were dedicated to each group. The solution obtained from fibroblast cell located in a 6-well plate and after placing the samples into the sink plate, RPMI-1640 medium, 10% FBS, and the antibiotics streptomycin and penicillin were added to cultured cells. The culture plate was incubated in the CO2 incubator and was studied after 24 hours. Finally, the effect of tested cement on the induction of IL-6 was evaluated using the ELISA test method. The statistical analysis was performed using one-way ANOVA. Results: IL-6 Production was considerably different among the investigated groups (P<0.001). Harvard cement caused IL-1b releasing much more than the resin cement. Conclusion: Considering the limitation of this study, Harvard cement might be considered to have more cytotoxic potencies in comparison with the other tested materials.

Pameijer CH. A review of luting agents. International journal of dentistry. 2012;2012.

El-Mowafy O. The use of resin cements in restorative dentistry to overcome retention problems. Journal-Canadian Dental Association. 2001 Feb;67(2):97-102.

Ulker HE, Sengun A. Cytotoxicity evaluation of self adhesive composite resin cements by dentin barrier test on 3D pulp cells. European journal of dentistry. 2009 Apr;3(2):120.

Gerzina TM, Hume WR. Diffusion of monomers from bonding resin-resin composite combinations through dentine in vitro. Journal of dentistry. 1996 Jan 1;24(1-2):125-8.

Geurtsen W, Spahl W, Müller K, Leyhausen G. Aqueous extracts from dentin adhesives contain cytotoxic chemicals. Journal of Biomedical Materials Research: An Official Journal of The Society for Biomaterials, The Japanese Society for Biomaterials, and The Australian Society for Biomaterials and the Korean Society for Biomaterials. 1999;48(6):772-7.

de Souza Costa CA, Hebling J, Hanks CT. Current status of pulp capping with dentin adhesive systems: a review. Dental Materials. 2000 May 1;16(3):188-97.

Schweikl H, Hartmann A, Hiller KA, Spagnuolo G, Bolay C, Brockhoff G, Schmalz G. Inhibition of TEGDMA and HEMA-induced genotoxicity and cell cycle arrest by N-acetylcysteine. dental materials. 2007 Jun 1;23(6):688-95.

Demirci M, Hiller KA, Bosl C, Galler K, Schmalz G, Schweikl H. The induction of oxidative stress, cytotoxicity, and genotoxicity by dental adhesives. dental materials. 2008 Mar 1;24(3):362-71.

Moharamzadeh K, Brook I, Van Noort R. Biocompatibility of resin-based dental materials. Materials. 2009;2(2):514-48.

Kong N, Jiang T, Zhou Z, Fu J. Cytotoxicity of polymerized resin cements on human

dental pulp cells in vitro. Dental materials. 2009 Nov 1;25(11):1371-5.

Souza PP, Aranha AM, Hebling J, Giro EM, de Souza Costa CA. In vitro cytotoxicity and in vivo biocompatibility of contemporary resin-modified glass-ionomer cements. Dental materials. 2006 Sep 1;22(9):838-44.

de Lima Pereira SA, de Menezes FC, Rocha-Rodrigues DB, Alves JB. Pulp reactions in human teeth capped with self-etching or total-etching adhesive systems. Quintessence International. 2009 Jun 1;40(6).

Trubiani O, Cataldi A, De Angelis F, D’Arcangelo C, Caputi S. Overexpression of interleukin‐6 and‐8, cell growth inhibition and morphological changes in 2‐hydroxyethyl methacrylate‐treated human dental pulp mesenchymal stem cells. International endodontic journal. 2012 Jan;45(1):19-25..