Creative Commons License 2019 Volume 6 Issue 3

Comparison of the Effect of Two Groups of Resin Cements (Panavia F2 and Rely X Plus) and Zinc Phosphate Cement (Harvard) On the Induc-tion of IL-6 by Category L-929 Mouse Fibroblast


Elham Romoozi, Mohammadali Salehian, Mahasti Sahabi, Mandana Sattari, Elahe Kamali
Abstract

Introduction: Resin cement, despite its biocompatibility, has been extensively applied in restorative dentistry during recent years. The resin matrix comprises one or more ‘light’ co-monomer systems (e.g. HEMA) and ‘heavy’ monomer systems (including Bis-GMA) to decrease the monomers’ viscosity and to increase the bonding strength to dentine. Acrylates, mainly methacrylates, have been revealed to cause cytotoxic impacts. Objective: This investigation contrasted the impact of resin cement (Rely X Plus and Panavia F2) and zinc phosphate cement (Harvard) on the initiation of IL-6 by category L-929 mouse fibroblast. Method: One resin cement (Panavia F2), one resin ionomer cement (Rely X Plus) and Harvard cement (Zinc Phosphate) were tested. The cement was prepared in hollow glass tubes (inner diameter of 5 mm, the height of 2mm) and 10 samples were dedicated to each group. The solution obtained from fibroblast cell located in a 6-well plate and after placing the samples into the sink plate, RPMI-1640 medium, 10% FBS, and the antibiotics streptomycin and penicillin were added to cultured cells. The culture plate was incubated in the CO2 incubator and was studied after 24 hours. Finally, the effect of tested cement on the induction of IL-6 was evaluated using the ELISA test method. The statistical analysis was performed using one-way ANOVA. Results: IL-6 Production was considerably different among the investigated groups (P<0.001). Harvard cement caused IL-1b releasing much more than the resin cement. Conclusion: Considering the limitation of this study, Harvard cement might be considered to have more cytotoxic potencies in comparison with the other tested materials.


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