Creative Commons License 2019 Volume 6 Issue 2

Punica Granatum-Based Green Ethanolic Extract as Highly Effective and Eco-Friendly Larvicide, Repellent against Medically Important Mosqui-to Vectors

M. Baranitharan, V. Tamizhazhagan, K. Kovendan, S. Senthilmurugan

Background & objective: Mosquito vectors are living organisms that can transmit infectious diseases between human from animals to humans. It is bloodsucking insect that ingest disease-producing microorganisms during a blood meal from an infected host. The present investigation discovered that the larvicidal activity of ethanol extract of P. granatum showed most mortality among the opposite crude extracts. Methods: The chemical composition of P. granatum ethanolic extract was analyzed by gas chromatography-mass spectroscopy. A total of twenty five III instar larvae of An. stephensi and Cx. quinquefasciatus were exposed to various concentrations (50-250 ppm) in the laboratory by using the standard protocol described by WHO (2005). The repellent activity of P. granatum chemical compositions tested against 100 blood starved female mosquitoes of An. stephensi and Cx. quinquefasciatus using the protocol of WHO (1996). Results: In GC-MS analysis, a total of seven compounds were identified in the ethanolic extract composition, the main component was Methyl 4-piperidineacetate. Further, the LC50 and LC90 values were found to be 110.36 and 212.28 mg/L against Cx. quinquefasciatus. The repellent activity to be best and therefore the most activity was ascertained at 3.5 mg/cm2 concentration provided 100% protection up to 240 min against Cx. quinquefasciatus. Conclusion: The results clearly show confirmed that the presence of active compounds in leaf of P. granatum.


Mehlhorn H, Al-Rasheid KA, Al-Quraishy S, Abdel-Ghaffar F. Research and increase of expertise in arachno-entomology are urgently needed. Parasitol Res 2012; 110: 259–265.

Benelli G. Research in mosquito control: current challenges for a brighter future. Parasitol Res 2015; 114: 2801–2805.

Baranitharan M, Dhanasekaran S, Murugan K, Kovendan K, Gokulakrishnan J, Benelli G. Coleus aromaticus leaf extract fractions: A source ofnovel ovicides, larvicides and repellents against Anopheles, Aedes and Culex mosquito vectors?. Proc Safe Environ Prot 2017; 106: 23-33.

Panneerselvam C, Murugan K, Kovendan K, Mahesh Kumar P, Subramaniam J. Mosquito larvicidal and pupicidal activity of Euphorbia hirta Linn. (Family: Euphorbiacea) and Bacillus sphaericus against Anopheles stephensi Liston (Dipteral: Culicidae). Asian Pac J Trop Med 2013; 102-109.

Jayaprasad B, Sharavanan S, Veerakumar K. Effect of Chloroxylon swietenia Dc bark extracts against Culex quinquefasciatus, Aedes aegypti and Anopheles stephensi larvae. Parasitol Res 2015; 114: 4219-4223.

Baranitharan M, Dhanasekaran S, Murugan K, Kovendan K, Gokulakrishnan J. Chemical composition and laboratory investigation of Melissa officinalis essential oil against human malarial vector mosquito, Anopheles stephensi L. (Diptera: Culicidae). J Coast Life Med 2016; 4: 969-973.

World Health Organization. Malaria Fact sheet N° 94: 2014.

World Health Organization. Malaria. Fact sheet December 9: WHO Press, 2015.

Samba Shiva Daravath, Siddaiah Reddya Naik B. Molecular Characterization and Phylogenetic Analysis of Culex quinquefasciatus by DNA Barcoding. Adv Entomol 2015; 3: 118-124.

Baranitharan M, Dhanasekaran S, Gokulakrishnan J, Krishnappa K, Deepa J. Mosquito larvicidal properties of Sesanum indicum L. against Aedes aegypti (Linn.), Anopheles stephensi (Liston), Culex quinquefasciatus (Say) (Diptera: Culicidae). Life Sci Archi 2015; 1: 72-77.

Ghosh S, Samanta A, Kole S. Mass durg administration for 1. elimination of lymphatic filariases: Recent experences from a district of West Bengal, India. J Trop Parasitol 2013; 3: 67-71.

Baranitharan M, Dhanasekaran S, Murugan K, Kovendan K, Gokulakrishnan J, Jeyasankar A. Experimental investigations of Nagapattinam indigenous medicinal plant extracts against dengue, malaria and filarial disease. Int J Zool Appl Biosci 2017; 2: 155-161.

World Health Organization. Lymphatic filariasis,” Fact sheet N°102: 2014.

Patil PB, Kallapur SV, Kallapur VL, Holihosur SN. Clerodendron inerme Gaertn. plants as an effective natural product against dengue and filarial vector mosquitoes. Asian Pac J Trop Dis 2014; 4: 453-462.

Dhanasekaran S, Krishnappa K, Anandan A, Elumalai K. Larvicidal, ovicidal and repellent activity of selected indigenous medicinal plants against malarial vector Anopheles stephensi (Liston), dengue vector Aedes aegypti (Linn.), Japanese encephalitis vector, Culex tritaeniorynchus (Giles.) (Diptera: Culicidae). J Agricul Technol 2013; 9: 29-47.

Murugan K, Dinesh D, Paulpandi M, Meqbel Althbyani AD, Subramaniam J, Madhiyazhagan P et al. Erratum to: Nanoparticles in the fight against mosquito-borne diseases: bioactivity of Bruguiera cylindrica-synthesized nanoparticles against dengue virus DEN-2 (in vitro) and its mosquito vector Aedes aegypti (Diptera: Culicidae). Parasitol Res 2015; 114: 4743.

Baranitharan M, Dhanasekaran S, Gokulakrishnan J, Mahesh Babu S, Thushimenan S. Nagapattinam medicinal plants against the dengue fever mosquito, Aedes aegypti. Int J Mosq Res 2016; 3: 29-34.

Benelli G. Plant-mediated biosynthesis of nanoparticles as an emerging tool against mosquitoes of medical and veterinary importance: a review. Parasitol Res 2016; 115: 23–34.

Senthilkumar A, Kannathasan K, Venkatesalu V. Chemical constituents and larvicidal property of the essential oil of Blumea mollis (D. Don) Merr. against Culex quinquefasciatus. Parasitol Res 2008; 959-962.

Govindaraju Ramkumar, Sengodan Karthi, Ranganathan Muthusamy, Devarajan Natarajan, Muthugounder Subramanian Shivakumar. Isecticidal and repellent activity of Clausena dentate (Rutaceae) plant extracts against Aedes aegypti and Culex quinquefasciatus mosquitoes (Diptera: Culicidae). Parasitol Res 2015; 114: 1139-1144.

Baranitharan M, Dhanasekaran S, Jeyasankar A, Arivoli S, Gokulakrishnan J. Studies on mossquitocidal activity of Ficus racemosa L. extracts. World J Pharmaceu Life Sci 2016; 2: 199-208.

Omena MC, Navarro DMAF, Paula FE, Luna JS, Lima MR, Sant’Ana AEG. Larvicidal activities against Ae. aegypti of some Brazilian medicinal plants. Bioresour Technol 2007; 98: 2549-2556.

Deepa J, Gokulakrishnan J, Baranitharan M, Dhanasekaran S. Larvicidal activity of Indian medicinal plants on the dengue fever mosquito, Aedes aegypti Linnaeus. Int J Pure Appl Zool 2015; 3: 130-136.

Lye C. Pomegranate: preliminary assessment of the potential for an Australian industry. Rural Industries Research and Development Corporation of Australian Government 2008; 17: 08/153.

Ageel AM, Al-Said MS, Al-Yhya AM, Islam MW, Tariq M. Effect of Salvia nematodes on sexual behavior of male rats. J Ethnopharmacol 1991; 33: 67-72.

Harlan JR. Crops and Man. 2nd Edition, American Society of Agronomy and Crop Science Society of America, Madison. WI 1992; 289.

Levin GM. Pomegranate (Punica granatum) plant genetic resources in Turkmenistan. Plant Genet Resour Newslet 1994; 97: 31-36.

Simmonds NW. Evolution of Crop Plants. Longman Editions, London, UK 1976; 340.

Melgarejo P, Salazar DM, Artés F. Organic acids and sugars composition of harvested pomegranate fruits. Europ Food Res Technol 2000; 211: 185-190.

Melgarejo P, Martínez J, Hernández F, Martínez FR, Barrows P, Erez A. Kaolin treatment to reduce pomegranate sunburn. Scientia Horticult 2004; 100: 349-353.

Mutahar S, Shiban M, Al-Otaibi M, Najeeb S, Al-Zoreky N. Antioxidant activity of pomegranate (Punica granatum L.) fruit peels. Food Nut Sci 2012; 3: 991-996.

Al-Zoreky NS. Antimicrobial activity of pomegranate (Punica granatum L.) fruit peels. Int J Food Microbiol 2009; 134: 244-248.

Voravuthikunchai SP, Limsuwan S, Supapol O, Subhadhirasakul S. Antibacterial activity of extract from family Zingiberaceae against food borne pathogens. J Food Saf 2006; 26: 325-334.

Tripathi SM, Singh DK. Molluscicidal activity of Punica granatum bark, Canna indica root. Brazilian J Med Biol Res 2000; 33: 1351-1355.

Gandhi N, Pillai S, Patel P. Efficacy of pulverized leaves of Punica granatum (Lythraceae) and Murraya koenigii (Rutaceae) against stored grain pest, Tribolium castaneum (Herbst.) (Coleoptera: Tenebrionidae). Int J Agricult Biol 2010; 12: 616-620.

Lakshmanan, S., S. Thushimenan, and V. Tamizhazhagan (2017). "Antifeedant, larvicidal and oviposition detergent activity of Pongamia pinnata and Ceiba pentandra against pod borer larvae of Helicoverpa armigera (Noctuidae: Lepidoptera)." Indo American Journal of Pharmaceutical Sciences 4.2 180-185

Kumaravel S, Praveen Kumar P, Vasuki P. GC-MS study on microbial degradation of Lindane. Int J Appl Chem 2010; 6: 363-366.

World Health Organization. Guidelines for laboratory and field testing of mosquito larvicides. Communicable disease control, prevention and eradication, WHO pesticide evaluation scheme. WHO, Geneva. WHO/CDS/WHOPES/GCDPP 2005; 1.3.

Finney DJ. A ststistical treatment of the sigmoid response curve. In: Probit analysis. Cambridge University Press, London 1971; 633.

Mathalaimuthu Baranitharan, Vairakannu Tamizhazhagan, Kalimuthu Kovendan (2019). Medicinal Plants as Potent Power for Malaria Control: Review, Entomology and Applied Science Letters, 5(1), 28-44.

Tamizhazhagan, K Pugazhendy, M Baranitharan, Humphrey Agevi (2017). Pest potential of Pisonia alba extracts and fractions against mosquito-borne disease (Diptera: Culicidae). International Journal of Pharma Sciences and Research.8, 5; 52-58.

Abbott WS. A method of computing the effectiveness of an insecticide. J Econ Entomol 1925; 18: 265-267.

World Health Organization. Guidelines for efficacy testing of mosquito repellents for human skins. WHO/HTM/NTD/WHOPES 2009; 4: 4-18.

Balu S, Gokulakrishnan J, Elanchezhiyan K, Deepa J. Mosquito larvicidal, ovicidal and pupicidal activities of Annona reticulata linn (Annonaceae) against Aedes aegypti (Linn.), Anopheles stephensi Liston and Culex quinquefasciatus (Say) (Diptera: Culicidae). Int J Rec Sci Res 2015; 6: 2690-2696.

Benelli G. Plant-mediated synthesis of nanoparticles: A newer and safer tool against mosquito-borne diseases? Asia Pac J Trop Biomed 2016; 6: 353-354.

Baranitharan, M., and Dhanasekaran, S. Evaluation of larvicidal activity of Ageratina adinophora (Spreng)King & H.Rob against Culex quinquefasciatus (Say). Int. J. Pharmaceu. Biol. Archie. 2014; 5: 120-125.

Jayanthi P, Lalitha P, Aarthi N. Larvicidal and pupicidal activity of extracts and fractionates of Eichhornia crassipes (Mart.) Solms against the filarial vector Culex quinquefasciatus Say. Parasitol Res 2012; 111: 2129-2135.

Ombito O Japheth, Matasyoh C Josphat, Vulule M John. Chemical composition and larvicidal activity of Zanthoxylum gilletii essential oil against Anopheles gambiae. African. J Biotechnol 2014; 13: 2175-2180.

Pavela R, Kaffkova K And Kumsta M. Chemical composition and larvicidal activity of essential oils from different Mentha L. and Pulegium species against Culex quinquefasciatus Say (Diptera: Culicidae). Plant Protect Sci 2014; 50: 36-42.

Govindarajan M, Rajeswary M, Hoti SL, Bhattacharyya A, Benelli G. Eugenol, α-pinene and β-caryophyllene from Plectranthus barbatus essential oil as eco-friendly larvicides against malaria, dengue and Japanese encephalitis mosquito vectors. Parasitol Res 2016; 115: 807-815.

Cheng SS, Huang CG, Chen YJ, Yu JJ, Chen WJ, Chang ST. Chemical compositions and larvicidal activities of leaf essential oils from two Eucalyptus species. Bioresour Technol 2009; 100: 452–456.

Khanna VG, Kannabiran K, Rajakumar G, Rahuman AA, Santhoshkumar T. Biolarvicidal compound gymnemagenol isolated from leaf extract of miracle fruit plant, Gymnema sylvestre (Retz) Schult against malaria and filariasis vectors. Parasitol Res 2011; doi:10.1007/s00436-011-2384-6.

Govindarajan M. Chemical composition and larvicidal activity of leaf essential oil from Clausena anisata (Willd.) Hook. f. ex Benth (Rutaceae) against three mosquito species. Asia Pac J Trop Med 2010; 3: 874–877.

Swati Supare, Mansi Patil. Estimation of phytochemical components from Cassia tora and to study its larvicidal activity. Int J Pharmaceu Sci Inv 2015; 4: 11-16.

Pohilt AM, Rezende AR, Lopes Baldin EL, Lopes NP, de Andrade Neto VF. Plant extracts, isolated phytochemicals, and plant-derived agents which are lethal to arthropod vectors of human tropical diseases—a review. Planta Med 2011; 77: 618–630.

Govindarajan M. Chemical composition and mosquitocidal potential of Mentha spicata (Linn.) essential. In: Govil JN, Bhattacharya S (eds) Recent progress in medicinal plants: essential oil III and phytopharmacology. Studium, USA, 2013; 153–172.

Sathya Narayanan PC, Kalpana Devi R, Pushpalatha M, Raveen R and Lingathurai S. Bio-Efficacy of Terminalia chebula Retz. (Combretaceae) against Culex quinquefasciatus Say (Diptera: Culicidae). Int J Curr Res Bioscie Plan Biol 2014; 3: 41-49.

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Entomology and Applied Science Letters supports the submission of entomological papers that contain information of interest to a wider reader groups e. g. papers bearing on taxonomy, phylogeny, biodiversity, ecology, systematic, agriculture, morphology. The selection of comprehensive, revisionary or integrated systematics studies of broader biological or zoogeographical relevance is also important. Distinguished entomologists drawn from different parts of the world serve as honorary members of the Editorial Board. The journal encompasses all the varied aspects of entomological research.