Creative Commons License 2018 Volume 5 Issue 3

Migalomorphic Spiders Venom: Extraction and Investigation of Biological Activity

Nagdalian A. Ashotovich, Pushkin S. Viktorovich, Povetkin S. Nikolaevich, Kopchekchi M. Egorovna, Marinicheva M. Petrovna, Lopteva M. Sergeevna

A number of researchers have noted that spiders produce a venom consisting of a mixture of potent selective toxins of different nature (from salts to large multi-domain proteins), each of which can have its own specific biological activity. A wide range of similar substances has been presented in venom of spiders which are an admixture of potent and selective toxins, each of which can have a specific biological activity. Considering the action mechanisms of venom, it is logical to assume that among the components of a poison of spiders, the peptides possessing antimicrobial activity can be found. From this point of view, mygalomorph spiders have been the most interesting to be studied. 24 mygalomorph spiders from 12 genera were studied to identify the influence of electrical stimulation parameters on the venom extracting. Electrical stimulation with various parameters allowed determining the spiders with the best ability of venom extracting. The selected venom was studied for insecticide and bactericide activity.  


Beisswenger, C. and Bals, R. Functions of antimicrobial peptides in host defense and immunity //Curr Protein Pept Sci. 2005. Vol. 6. № 3. P. 255-264.

Bucherl, W.: Studies on dried venom of PhoneutriaferaPerty, 1833. In: Venoms. Ed E.E. Buckley and N. Porges. Washington, D.C.: Amer. Ass. Advanc. Sci 1956 pp. 95-97.Google Scholar

Bucherl, W.: Spiders. In: Venomous Animals and Their Venoms. Ed. W. Bucherl and E.E. Buckley New York-London, Academic Press 1971, Vol. III, pp. 197–277.

Database of polypeptide sequences UniProt (Universal Protein Resource): mmm.

Kozlov, S.A., Vassilevski, A.A., Grishin, E.V. (2008) in ''Peptidomics: methods and applications'' (Soloviev, M., Shaw, C., Andrén, P., Eds.). Ho boken: John Wiley & Sons. pp. 55–70.

Selimov, M. A. Features of the study of the morphology of abnormal forms of red blood cells by atomic force microscopy. Selimov, M. A., Demchenkov E. L., A. A. Naghdalyan, Gatina J. S. Science. Innovations. Technologies. 2015. No. 3. P. 145-158.

Sollod BL, Wilson D, Zhaxybayeva O, Gogarten JP, Drinkwater R, King GF (2005) Were arachnids the first to use combinatorial peptide libraries? Peptides 26, 131–139.

Vassilevski A, Kozlov S, Grishin E V (2009) Molecular diversity of spider venom. Biochem. 74, 1505–34.

Kuhn-Nentwig L, Schaller J, Nentwig W (1994) Purification of toxic peptides and the amino acid sequence of CSTX-1 from the multicomponent venom of Cupienniussalei (Araneae:Ctenidae). Toxicon 32, 287–302.

Shlyapnikov YM, Andreev Y, Kozlov S, Vassilevski A, Grishin E V (2008) Bacterial production of latarcin 2a, a potent antimicrobial peptide from spider venom. ProteinExpr. Purif. 60, 89–95.

Li Y (2011) Recombinant production of antimicrobial peptides in Escherichia coli: a review. ProteinExpr. Purif. 80, 260–7.

Vassilevski A, Kozlov S, Egorov TA, Grishin E V (2010) Purification and characterization of biologically active peptides from spider venoms. MethodsMol. Biol. 615, 87–100

Garb JE, Hayashi CY (2013) Molecular evolution of α-latrotoxin, the exceptionally potent vertebrate neurotoxin in black widow spider venom. Mol. Biol. Evol. 30, 999–1014

Jiang L, Liu C, Duan Z, Deng M, Tang X, Liang S (2013) Transcriptome analysis of venom glands from a single fishing spider Dolomedesmizhoanus. Toxicon 73, 23–32.

Diego-García E, Peigneur S, Waelkens E, Debaveye S, Tytgat J (2010) Venom components from Citharischiuscrawshayi spider (Family Theraphosidae): exploring transcriptome, venomics, and function. Cell. Mol. LifeSci. 67, 2799–813.

Zhang Y, Chen J, Tang X, Wang F, Jiang L, Xiong X, Wang M, Rong M, Liu Z, Liang S (2010) Transcriptome analysis of the venom glands of the Chinese wolf spider Lycosasingoriensis. Zoology (Jena). 113, 10–8.

Fernandes-Pedrosa M de F, Junqueira-de-Azevedo I de LM, Gonçalves-de-Andrade RM, Kobashi LS, Almeida DD, Ho PL, Tambourgi D V (2008) Transcriptome analysis of Loxosceleslaeta (Araneae, Sicariidae) spider venomous gland using expressed sequence tags. BMC Genomics 9, 279.

Cordes MHJ, Binford GJ (2006) Lateral gene transfer of a dermonecrotic toxin between spiders and bacteria. Bioinformatics 22, 264–8.

Tang X, Zhang Y, Hu W, Xu D, Tao H, Yang X, Li Y, Jiang L, Liang S (2010) Molecular diversification of peptide toxins from the tarantula Haplopelmahainanum (Ornithoctonushainana) venom based on transcriptomic, peptidomic, and genomic analyses. J. ProteomeRes. 9, 2550–2564.

Kozlov S, Malyavka A, McCutchen B, Lu A, Schepers E, Herrmann R, Grishin E (2005) A novel strategy for the identification of toxinlike structures in spider venom. Proteins 59, 131–40.

Krapcho KJ, Kral Jr. RM, Vanwagenen BC, Eppler KG, Morgan TK, Kral RM (1995) Characterization and cloning of insecticidal peptides from the primitive weaving spider Diguetiacanities. InsectBiochem. Mol. Biol. 25, 991–1000.

Kiyatkin NI, Kulikovskaya IM, Grishin E V, Beadle DJ, King LA (1995) Functional characterization of black widow spider neurotoxins synthesised in insect cells. Eur. J. Biochem. 230, 854–9.

Vassilevski A, Kozlov S, Grishin E V (2008) Antimicrobial peptide precursor structures suggest effective production strategies. RecentPat. Inflamm. AllergyDrugDiscov. 2, 58–63.

Vassilevski A, Fedorova IM, Maleeva EE, Korolkova Y V, Efimova SS, Samsonova O V, Schagina L V, Feofanov A, Magazanik LG, Grishin E V (2010) Novel class of spider toxin: active principle from the yellow sac spider Cheiracanthiumpunctorium venom is a unique two-domain polypeptide. J. Biol. Chem. 285, 32293–302.

Lazarev VN, Shkarupeta MM, Polina NF, Kostrjukova ES, Vassilevski A, Kozlov S, Grishin E V, Govorun VM (2013) Antimicrobial peptide from spider venom inhibits Chlamydia trachomatis infection at an early stage. Arch. Microbiol. 195, 173–9.

Vassilevski A, Kozlov S, Samsonova OVO, Egorova NNS, Karpunin DVD, Pluzhnikov KKA, Feofanov A, Grishin EVE (2008) Cyto-insectotoxins, a novel class of cytolytic and insecticidal peptides from spider venom. Biochem. J. 696, 687–696.

Won A, Ruscito A, Ianoul A (2012) Imaging the membrane lytic activity of bioactive peptide latarcin 2a. Biochim. Biophys. Acta 1818, 3072–80

Polyansky AA, Vassilevski A, Volynsky PE, Vorontsova O V, Samsonova O V, Egorova NS, Krylov NA, Feofanov A, Arseniev AS, Grishin E V, Efremov RG (2009) N-terminal amphipathic helix as a trigger of hemolytic activity in antimicrobial peptides: a case study in latarcins. FEBS Lett. 583, 2425–8.

Kozlov S, Vassilevski A, Feofanov A, Surovoy AY, Karpunin DV, Grishin EV (2006) Latarcins, antimicrobial and cytolytic peptides from the venom of the spider Lachesanatarabaevi (Zodariidae) that exemplify biomolecular diversity. J. Biol. Chem. 281, 20983–92

Grishin E V, Volkova TM, Arseniev AS (1989) Isolation and structure analysis of components from venom of the spider Argiopelobata. Toxicon 27, 541–9.

Vassilevski A.A., Sachkova M.Y., Ignatova A.A., Kozlov S.A., Feofanov A.V., Grishin E.V. Spider toxins comprising disulfide-rich and linear amphipathic domains: a new class of molecules identified in the lynx spider Oxyopestakobius. FEBS J. 2013, 280, 6247–6261.

Sachkova M.Y., Slavokhotova A.A., Grishin E.V., Vassilevski A.A. Genes and evolution of two-domain toxins from lynx spider venom. FEBS Lett. 2014, 588, 740–745.

Sachkova, M.Y., Slavokhotova, A.A., Grishin, E.V., Vassilevski A.A. Structure of the yellow sac spider Cheiracanthiumpunctorium genes provides clues to evolution of insecticidal two-domain knottin toxins. Insect Mol. Biol. 2014, doi: 10.1111/imb.12097.

Rabia Yaqoob, Hafiz Muhammad Tahir, Muhammad Arshad, Sajida Naseem.Optimization of the Conditions for Maximum Recovery of Venom from Scorpions by Electrical Stimulation. Pakistan J. Zool., 2016, vol. 48(1), pp. 265-269.

Nisani, Z., Dunbar, S.G. andHayes, W. K., 2007. Cost of venom regeneration in Parabuthustransvaalicus(Arachnida: Buthidae). Comp. Biochem. Physiol., 147: 509–513.

Kharchenko, L.N., Pushkin, S.V. Biocenotic and practical value of spiders (Arachnida, Aranei) // Fauna of Stavropol. Volume 7: SSU, 1999. P.120-125.

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