2017 Volume 4 Issue 2

The Sensitivity of the Real-time PCR and Nested-PCR for Detection of Coxiella burnetii in Milk Samples

Mojtaba Bonyadian, Hamdollah Moshtaghi, Hamidreza Kazemeini
Coxiella burnetii is the causative agent of Q-fever, a widespread zoonosis. In domestic animals infection remains either asymptomatic or presents as infertility or abortion. Clinical presentation in humans can range from mild flu-like illness to acute pneumonia and hepatitis. In humans serology is the gold standard for diagnosis but is inadequate for early case detection, so real-time PCR and nested-PCR assays were developed in this study to measure amounts of C.burnetii shed in milk. Our study was to assess the sensitivity of the realtime PCR and nested-PCR for detection of Coxiella burnetii in bovine bulk milk samples from dairy herds in 3 provinces (Chaharmahal and Bakhtiari , Isfahan and Yazd) of Iran. In the present study, 300 bulk milk samples from 89 dairy cattle herds were tested for C. burnetii using real-time PCR and nested-PCR assays. The animals which their milk samples collected for this study were clinically healthy. In total, 74 of 300 (24.7%) cow milk samples were positive in real-time PCR assay and 26 of 300 (8.7%) samples were positive in nested-PCR assay. McNemar test shows a significant difference in detection of C. burnetii between real-time PCR and nested-PCR. Also the results of this study indicate those clinically healthy dairy cows are important sources of C. burnetii infection in Iran.


Maurin M, Raoult D. Q fever. Clin Microbiol Rev 1999; 12: 518–53.

Hirai K, To H. J Vet Med Sci 1998; 60: 781–90.

Babudieri B. Adv Vet Sci 1959; 5:81–182.

Htwe KK, Amano K, Sugiyama Y, Yagami K, Minamoto N, Hashimoto A, et al. Vet Rec 1992; 131:490. [5] Lang GH. Can J Public Health 1988; 79: 56–9.

Wisniewski H, Krumbiegel ER. Arch Environ Health 1970; 21: 58–62.

Lang GH. Coxiellosis (Q fever) in animals. In: T.J. Marrie, editor. Q fever, volI. The disease. Boca Raton (FL): CRC Press, 1990. pp23–48.

McQuiston JH, Childs JE. Vector Borne Zoonotic Dis 2002; 2: 179–91. [9] Hoover TA, Vodkin MH, Williams JC. J Bacteriol 1992; 174: 5540–8.

Willems H, Thiele D, Frolich-Ritter R, Krauss H. J Vet Med B 1994;41: 580–7.

Luoto L. Public Health Rep 1960;75: 135– 40. [12] Biberstein EL, Behymer DE, Bushnell R, Crenshaw G, Riemann HP, Franti CE. Am J Vet Res 1974;12: 1577–2.

Ghalyanchi Langeroudi A, Raees Babakhani N, Zolfaghari MR ,Majidzadeh KA, Morovvati A, Soleimani M, IJVM 2013; 7: 207-211.

Kazar J, Brezina R, Palaova P. Bull WHO 1982;60: 389-394.

Guatteo R, Beaudeau F, Joly A, Seegers H. Zoonoses and Public Health 2007; 54: 191-194.

Arricau-Bouvery N, Rodolakis A .Is Q fever an emerging or reemerging zoonosis? Vet. Res. 2005;36: 327-349.

Zhang GQ, Nguyen SV, To H, Ogawa M, Hotta A, Yamaguchi T, Kim HJ, Fukushi H, Hirai K. J Clin Microbiol 1998; 36: 77-80.

Addo PB . Br. Vet. J 1980; 136: 519-521.

Soliman AK, Botros BA, Watts DM. J. Clin. Microbiol 1992; 30: 1595-1597.

Rodolakis A, Berri M, Héchard C, Caudron C, Souriau A, Bodier CC, Blanchard B, Camuset P, Devillechaise P, Natorp JC, Vadet JP, ArricauBouvery N. J. Dairy Sci 2007;90: 5352-5360.

Berri M, Arricau-Bouvery N, Rodolakis A. PCR-based detection of Coxiella burnetii from clinical samples. In: Sachse K., Frey J. (Eds.), Methods in molecular biology, Humana Press Inc., Totowa, NJ ,2003. pp. 153-161.

Öngör H, Cetinkaya B, Karahan M, Nuri Acik M, Bulut H, Muz A. Vet. Rec 2004; 154: 570-572.

Fretz R, Schaeren W, Tanner M, A. Baumgartner. Int. J. Food Microbiol 2007; 116: 414-418.

Entomology and Applied Science Letters is an international peer reviewed publication which publishes scientific research & review articles related to insects that contain information of interest to a wider audience, e.g. papers bearing on the theoretical, genetic, agricultural, medical and biodiversity issues. Emphasis is also placed on the selection of comprehensive, revisionary or integrated systematics studies of broader biological or zoogeographical relevance. Papers on non-insect groups are no longer accepted. In addition to full-length research articles and reviews, the journal publishes interpretive articles in a Forum section, Short Communications, and Letters to the Editor. The journal publishes reports on all phases of medical entomology and medical acarology, including the systematics and biology of insects, acarines, and other arthropods of public health and veterinary significance.
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