2017 Volume 4 Issue 1

Assessment of post mortem epididymal sperm from non-descript bucklings (Capra hircus)

Maureen B. Gajeto, Julius V. Tomas, Kristina J. Cruz, Marlon B. Ocampo, Lerma C. Ocampo
Recent progress  in gamete preservation have utilized epididymal sperm for animal genetic resource cryobanking of traditional livestock breeds and endangered animals not trained for semen collection. Therefore, appropriate analysis to ensure its quality is paramount. In this study, post mortem epididymal sperm of bucklings (n=8) were evaluated to determine its possible use in related assisted reproductive techniques. The epididymides were sliced longitudinally and the sperm collected through swim-up method. Significant variations on semen quality and quantity among individual buckling were observed. The mean volume recovered was0.65 ml with 6.3 pH. The sperm motility ranged from 50-75% with a mean concentration of 1.92×109 cells/ml. The percentage viability was ≥80% with percentage normality range of 48.95-82.2%. The common head and tail abnormalities were the small, pyriform type and cytoplasmic droplets in the proximal region. The results showed that the epididymides of buckling contain a significant population of normal sperm that could be used for fertilization studies both  in vivo or in vitro.


M.A.F. Bertol, R.R. Weiss, V. Thomaz-Soccol, L.E. Kozicki, A.S. Fujita, R. Azebedo de Abreu, K.T. Green, Brazilian Arch Biol Tech, 2013.56, 5. [2] J.A. Bezerra, A.M. da Silva, G.C. Peixoto, M.A. da Silva, M. Franco de Oliveira, A.R. Silva, Zool Sci, 2014.31, 338-342.

J.T. Carreira, G.Z. Mingoti, L.H. Rodriguez, C. Silva, S.H. Perri, M.B. Koivisto, Acta Vet Scandinavia, 2012.54, 1-7.

R. Foote, J Androl, 2000.21, 355.

S. Fetic, C. Yeung, B. Sonntag, E. Nieschlag, T.G. Cooper, J Androl, 2006.27, 294-301.

O. Garcia-Alvarez, A. Maroto-Morales, F.M. Pastor, J.J. Garde, M. Ramon, M.F. Santos, M.C. Esteso, M.P. Guzman, A.J. Soler, Theriogenology, 2009.72, 160-168.

D. Gerber, P.C. Irons, A. Arlotto, D. Cooper, Theriogenology, 2001.55, 384.

F. Jimenez, J.S. Vicente, M.P. Viudes-de-Castro, Reprod Dom Anim, 2008.43, 403-408.

S.K. Jindal, J.N. Panda, J Reprod Fertil, 1980. 59, 469-471.

M. Kaabi, P. Paz, M. Alvarez, E. Anel, J.C. Boixo, H. Rouissi, P. Herraez, L. Anel, Theriogenology, 2003.60, 1249-1259.

K. Kikuchi, T. Nagai, N. Kashiwazaki, H. Ikeda, J. Noguchi, A. Shimada, E. Soloy, H. Kaneko, Theriogenology, 1998. 50, 615-623.

N.W.K. Karja, E.M.A. Respaty, I. Nuraini, S.A. S. Parino, ,J Indonesian Trop Anim Agric, 2010. 35, 63-67.

K. Lubbe, P. Bartels, I. Kilian, Y. Friedmann, R.A. Godke, Theriogenology, 2000. 53, 338.

F.V. Mamuad, E.V. Venturina, H. Saito, Collection, processing and handling of buffalo semen. Water Buffaloes and Beef Cattle Improvement Project. A joint-JICA Assisted Project of the Philippine Carabao Center and the Bureau of Animal Inductry, Philippines, 2004. p. 1-60

F. Martinez-Pastor, C. Guerra, M. Kaabi, A.R. Diaz, E. Anel, P. Herraez, P. de Paz, L. Anel, Theriogenology, 2005a,63, 24-40.

F.C. Molinia, G. Evans, C. Quintana, W.M.C. Maxwell, Anim Reprod Sci, 1994.36, 113-122.

J.S. Moreno, M.A. Coloma, J. Dorado, A.P. Pastor, F. Gomez, R. Vega, A. Brunet, L.A. Sebastian, Theriogenology, 2009.71, 12531260.

G.S. Prins, Encyclopedia of Reprod, 1999.4, 360-367. [19] P.H. Purdy, Small Rumin Res, 2006.63, 215225.

F.V. Ramukhithi, T.L. Nedambale, B. Sutherland, K.C. Lehloenya, African J Biotech, 2011.10, 17898-17902.

S. K. Sani, F. Barati, M.K. Mahabady, Iran J Reprod Med, 2013.11, 747-752.

E. Shokrollahi, F. Barati, S. Gooraninejad, Small Rumin Res, 2014.119, 96-99.

M.A. Silva, G.C. Peixoto, E.A. Santos, I.S. Castelo, M.F. Oliveira, A.R. Silva, Theriogenology, 2011. 76, 1084-1089.

F. Turri, M. Madeddu, T.M. Gliozzi, G. Gandini, F. Pizzi, Reprod Dom Anim, 2011.47, 712-717.

F. Turri F, M. Madeddu, T.M. Gliozzi, G. Gandini, F. Pizzi, Animal, 2013. 88, 440-447

S.W. Walkden-Brown, B.J. Restall, W.A. Taylor, Reprod Fertil Dev,1994,6, 727-736.

H. Xu, S.Q. Yuan, Z.H. Zheng, W. Yan, Asian J Androl, 2013.15, 799-805.

Y. Yulnawati, H. Maheshwari, Herdis, M. Rizal, Biotropia, 2009.16, 21-27.

Y. Yulnawati, H.Gunawan, H. Maheshwari, M. Rizal, Herdis, A. Boediono, Hayati J BioSci, 2010.17, 27-30.

Y. Yulnawati, M. Rizal, H. Maheshwari, R.R. Noor, C. Sumantri, A. Boediono, JITV, 2013a.18, 202-207.

Y. Yulnawati, H. Maheshwari, M. Rizal, A. Boediono, Media Peternakan, 2013b. 36, 101105.

N. Nasir, A.A. Njidda, A.M. Hassan, J Sci, 2014.4, 250-254.

Entomology and Applied Science Letters is an international peer reviewed publication which publishes scientific research & review articles related to insects that contain information of interest to a wider audience, e.g. papers bearing on the theoretical, genetic, agricultural, medical and biodiversity issues. Emphasis is also placed on the selection of comprehensive, revisionary or integrated systematics studies of broader biological or zoogeographical relevance. Papers on non-insect groups are no longer accepted. In addition to full-length research articles and reviews, the journal publishes interpretive articles in a Forum section, Short Communications, and Letters to the Editor. The journal publishes reports on all phases of medical entomology and medical acarology, including the systematics and biology of insects, acarines, and other arthropods of public health and veterinary significance.
Issue 1 Volume 8 - 2021
Call for Papers
Entomology and Applied Science Letters supports the submission of entomological papers that contain information of interest to a wider reader groups e. g. papers bearing on taxonomy, phylogeny, biodiversity, ecology, systematic, agriculture, morphology. The selection of comprehensive, revisionary or integrated systematics studies of broader biological or zoogeographical relevance is also important. Distinguished entomologists drawn from different parts of the world serve as honorary members of the Editorial Board. The journal encompasses all the varied aspects of entomological research. This has become the need felt in scientific research due to the emphasis on intra-, inter-, and multi-disciplinary approach.