2017 Volume 4 Issue 2

Viability of carabao (Bubalus bubalis carabanensis) epididymal sperm from post mortem testes in semen extender at refrigerated temperature

Lean Angelo B. San Diego, Marvin Bryan S. Salinas, Angelica C. Bumanlag, Marlon B. Ocampo, Lerma C. Ocampo
Post mortem epididymal sperm (ES) is an excellent source of germplasm for conservation of animal genetic resources. In this study,  the viability of post mortem ES from carabao after collection at ambient temperature (AT) and after 24 hr and 48 hr of storage at refrigeration temperature (RT) were evaluated. ES were collected through slicing of epididymides from 7 carabaos. The mean ES volume was 0.4 ml with sperm concentration of 2.5×109 cells/ml. The mean percentage livability of fresh ES, after 24 hr and 48 hr of storage at RT were 81.93%, 65.93% and 43.7%, respectively. The mean percentage abnormalities of fresh ES, after 24 hr and 48 hr of storage at RT were 44.15%, 38.5% and 43.47%, respectively. The mean percentage motility of fresh ES, after 24 hr and 48 hr of storage at RT were 60.0%, 50.0% and 17.24%, respectively, after analysis through conventional means. Through CASA, the mean percentage motility was observed to be lower significantly at 26.12%, 37.94% and 18.32% for fresh ES, after 24 hr and 48 hr of storage at RT, respectively. The mean progressive motility of fresh ES, after 24 hr and 48 hr storage at RT were 10.02%, 16.47% and 10.87%, respectively. The results suggest that ES potential for use in fertilization studies remained viable when used immediately or after storage at RT for the first 24 hr.


F.P. Aquino, E.P. Atabay, E.C. Atabay, M.B. Ocampo, P.G. Duran, P.B. Pedro, D.H. Duran, R.V. De Vera, L.C. Cruz, Buffalo Bull, 2013. 32(2):545548.

L.M. Aquino, F.V. Manaois, L.C. Ocampo, M.B. Ocampo, Intl J Agric Tech, 2015.11:2531-2538.

E.P. Atabay, E.C. Atabay, P.G. Duran, E.B. Flores, A.F. Abesamis, R.V. De Vera, L.C. Cruz, Phil J Vet Anim Sci,2008.34:55-65.

M.A.F. Bertol, R.R. Weiss, V. Thomaz-Soccol, L.E. Kozicki, A.S. Fujita, R. Azebedo de Abreu, K.T. Green, Brazilian Arch Biol Tech, 2013. 56:5.

R. Boni, S. Roviello, B. Gasparrini, M. Langella, L. Zicarelli, Buffalo J, 1999.1:115-120.

F.P. Chatiza, G.M. Pieterse, P. Bartel, T.L. Nedambale, Anim Reprod Sci, 2011.126:234-244.

U. Chuangsoongneon, M. Kamonpatana, Buffalo J, 1991.7:189-198.

M.R. Jainudeen, Y. Takahashi, M. Nihayah, H. Kanagawa, Anim Reprod Sci, 1993.31:205-212.

S.K. Jindal, J.N. Panda, J Reprod Fert, 1980. 59:469-471

B. Gasparrini, Theriogenology, 2002.57:237256.

D. Gerber, P.C. Irons, A. Arlotto, D. Cooper, Theriogenology, 2001.55:384

M. Hishinuma, K. Suzuki, J. Sekine, Theriogenology, 2003.59:813-820. [13] D. Hufana-Duran, F.P. Aquino, R.V. De Vera, P.B. Pedro, L.C. Cruz, Korean J Anim Reprod, 1998.21:265-272.

D. Hufana-Duran, P.B. Pedro, H.V. Venturina, R.D. Hufana, A.L. Salazar, P.G. Duran, L.C. Cruz, Theriogenology, 2004.61:1429-1439.

D. Hufana-Duran, P.B. Pedro, A.L. Salazar, H.V. Venturina, P.G. Duran, L.C. Cruz, Phil J Sci,2008.137:99-104.

M. Kaabi, P. Paz, M. Alvarez, E. Anel, J.C. Boixo, H. Rouissi, P. Herraez, L. Anel, Theriogenology, 2003.60:1249-1259.

N.W.K. Karja, E.M.A. Respaty, I. Nuraini, S.A. Prihatno, S. Gustari, J Indonesian Trop Anim Agric, 2010.35:63-67.

F.V. Mamuad, E.V. Venturina, H. Saito, Collection, processing and handling of buffalosemen. Water Buffaloes and Beef Cattle Improvement Project. A joint-JICA Assisted Projectof the Philippine Carabao Center and the Bureau of Animal Industry, Philippines, 2004. 160.

F.V. Mamuad, E.V. Venturina, R.T. Morcoso, E.C. Atabay, K. Kudo, Manual for water buffaloes. WBBCIP, BAI, Philippines, 2005.1-45.

F. Martinez-Pastor, C. Guerra, M. Kaabi, A.R. Diaz, E. Anel, P. Herraez, P. de Paz, L. Anel, Theriogenology, 2005.63:24-40.

C.F. Martins, R. Rumpf, D.C. Pereira, M.N. Dode, Anim Reprod Sci, 2007.101:326-331.

L.C. Ocampo, M.B. Ocampo, F.P. Aquino, R.V. De Vera, L.C. Cruz, Proc 2nd ABA Cong.Manila, Philippines, 1996.2:412-416.

M.B. Ocampo, L.C. Ocampo, N.D. Lorenzo, F.V. Mamuad, F.P. Aquino, T. Mori, H. Shimizu, L.C. Cruz, Austr J Anim Sci, 2000. 13:279-281. [24] L.C. Ocampo, T. Mori, F.V. Mamuad, M.B. Ocampo, H. Shimizu, Bubalus bubalis; J Buff Sci Tech,2001.2:68-77.

L.C. Ocampo, F.V. Mamuad, T. Mori, H. Shimizu, M.B. Ocampo, Buffalo J, 2001.19:145154.

M.B. Ocampo, L.C. Ocampo, Intl J Agric Tech, 2015. 11:2343-2357.

L.C. Ocampo, L.M. Rigos, M.B. Ocampo, Res Opin Anim Vet Sci, 2016. 6:24-31.

L.C. Ocampo, E.F. Celestino, F.P. Aquino, M.B. Ocampo, Res Opin Anim Vet Sci, 2016.6:173-177.

E. Shokrollahi, F. Barati, S. Gooraninejad, Small Rum Res, 2014.119:96-99.

F. Turri, M. Madeddu, T.M. Gliozzi, G. Gandini, F. Pizzi, Reprod Dom Anim, 2011.47:712-717

F. Turri, M. Madeddu, T.M. Gliozzi, G. Gandini, F.Pizzi, Animal, 2014. 88:440-447

O. Varisli, C. Uguz, C. Agca, Y. Agca, Anim Reprod Sci, 2009. 110:256-268.

H.V. Venturina, F. Adriano, P. Duran, N.D. Lorenzo, O.F. Smith, L.C. Cruz, Phil J Anim Sci,1991. 17:55. [34] Yulnawati, H. Maheshwari, Herdis, M. Rizal, Biotropia, 2009.16(1):21-27.

Yulnawati, M. Gunawan, H. Maheshwari, M. Rizal, Herdis, A. Boediono, Hayati J Bio Sci, 2010.17(1):27-30.

Y. Yulnawati, M. Rizal, H. Maheshwari, R.R. Noor, C. Sumantri, A. Boediono, JITV, 2013.18 (3):202-207

Entomology and Applied Science Letters is an international peer reviewed publication which publishes scientific research & review articles related to insects that contain information of interest to a wider audience, e.g. papers bearing on the theoretical, genetic, agricultural, medical and biodiversity issues. Emphasis is also placed on the selection of comprehensive, revisionary or integrated systematics studies of broader biological or zoogeographical relevance. Papers on non-insect groups are no longer accepted. In addition to full-length research articles and reviews, the journal publishes interpretive articles in a Forum section, Short Communications, and Letters to the Editor. The journal publishes reports on all phases of medical entomology and medical acarology, including the systematics and biology of insects, acarines, and other arthropods of public health and veterinary significance.
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Entomology and Applied Science Letters supports the submission of entomological papers that contain information of interest to a wider reader groups e. g. papers bearing on taxonomy, phylogeny, biodiversity, ecology, systematic, agriculture, morphology. The selection of comprehensive, revisionary or integrated systematics studies of broader biological or zoogeographical relevance is also important. Distinguished entomologists drawn from different parts of the world serve as honorary members of the Editorial Board. The journal encompasses all the varied aspects of entomological research. This has become the need felt in scientific research due to the emphasis on intra-, inter-, and multi-disciplinary approach.