2016 Volume 3 Issue 5

Combined preconditioning with hypoxia and Hydrogen Peroxide Improved efficiency of Mesenchymal Stem Cell in cell culture


Shiva Khatibi, Zahra Farzaneh Taban, Raheleh Halabian, Amaneh Mohammadi Roushandeh
Abstract

Preconditioning with hypoxia or ROS such as hydrogen peroxidase improved long term survival of Mesenchymal Stem Cells. But combined therapy with both of them is not investigated enough in cell culture. Therefore, the present study investigates the protective effects of co-preconditioning of the cells with H2O2 and hypoxia in cell culture. Bone Marrow MSCs were cultured and divided in 6 groups; control, groups I and II exposed to H2O2, group III treated with COCL2, Groups IV and V treated withbothH2O2andCOCL2 during 6, 12, 24 and 48h. Then, all groups were underwent to lethal dose of H2O2 (300 μM) for 24 hours followed by 24h recovery. Afterward, MTT assay and trypan blue staining were conducted to evaluate the cell proliferation and viability. Also, TUNEL was done to study the cell apoptosis. Cell proliferation and viability after preconditioning with H2O2 and hypoxia were considerably increased compared to the control group after 6, 12, 24 and 48 hours (P < 0.01). Furthermore, cell proliferation and viability in groups IV and V were remarkably more than control and others preconditioning groups (P < 0.01). Apoptosis was significantly decreased in preconditioned groups compared to the control group after 12 (P < 0.01), 24 (P < 0.05) and 48 hours (P < 0.01). Also, cell apoptosis was decreased significantly in groups IV and V after 12, 24 and 48 hours compared to control and other preconditioning groups (P < 0.01). This study clearly demonstrated that preconditioning with H2O2 and COCL2 can improve BMMSCs proliferation and viability and also have inhibitory effect on cell apoptosis.

 

Keywords: Bone Marrow Mesenchymal Stem Cells, hypoxia, oxidative stress, co-preconditioning.


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Entomology and Applied Science Letters is an international peer reviewed publication which publishes scientific research & review articles related to insects that contain information of interest to a wider audience, e.g. papers bearing on the theoretical, genetic, agricultural, medical and biodiversity issues. Emphasis is also placed on the selection of comprehensive, revisionary or integrated systematics studies of broader biological or zoogeographical relevance. Papers on non-insect groups are no longer accepted. In addition to full-length research articles and reviews, the journal publishes interpretive articles in a Forum section, Short Communications, and Letters to the Editor. The journal publishes reports on all phases of medical entomology and medical acarology, including the systematics and biology of insects, acarines, and other arthropods of public health and veterinary significance.
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