2017 Volume 4 Issue 2

Sperm motility assessment of epididymal sperm from post mortem goat testicles held at 5°C

Angelica C. Bumanlag, Hannah Lei M. Harada, Cynthia C. Divina, Marlon B. Ocampo, Lerma C. Ocampo

Appropriate holding conditions for post mortem testicles of goat to yield quality epididymal sperm (ES) as a source of genetic material for cryobanking and fertilization studies are lacking. In this study, the effect of storage time on the motility of ES from post mortem testicles maintained at 5°C was evaluated. In the laboratory, the cauda epididymides were excised from the testicles after 4 hr (G-1) and 24 hr (G-2) of holding time before collecting the sperm in a Tris-citrate buffered solution and evaluated using a CASA. Sperm motility profiling revealed a subpopulation of static, slow, motile and progressive ES. The proportion of static sperm in the control (16.97±6.21) and G-1 (21.53±5.60) were lower significantly than G-2 (36.13±5.05). The proportion of slow moving sperm was lower significantly than G-1 (23.31±3.57) and G-2 (25.45±3.32). The proportion of motile and progressive motile sperm decreases significantly (P<0.05) as the holding time increases at 78.46±4.64% (G-1) to 63.85±4.06 (G-2) and 45.53±8.89 (G-1) to 25.46±8.42 (G-2), respectively. The results showed that prolonged storage of post mortem testicles at 5°C could result to a reduced percentage of motile and progressively motile ES. Nevertheless, this considerable proportion of ES remained useful both for cryobanking and fertilization studies.


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Entomology and Applied Science Letters is an international peer reviewed publication which publishes scientific research & review articles related to insects that contain information of interest to a wider audience, e.g. papers bearing on the theoretical, genetic, agricultural, medical and biodiversity issues. Emphasis is also placed on the selection of comprehensive, revisionary or integrated systematics studies of broader biological or zoogeographical relevance. Papers on non-insect groups are no longer accepted. In addition to full-length research articles and reviews, the journal publishes interpretive articles in a Forum section, Short Communications, and Letters to the Editor. The journal publishes reports on all phases of medical entomology and medical acarology, including the systematics and biology of insects, acarines, and other arthropods of public health and veterinary significance.
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Entomology and Applied Science Letters supports the submission of entomological papers that contain information of interest to a wider reader groups e. g. papers bearing on taxonomy, phylogeny, biodiversity, ecology, systematic, agriculture, morphology. The selection of comprehensive, revisionary or integrated systematics studies of broader biological or zoogeographical relevance is also important. Distinguished entomologists drawn from different parts of the world serve as honorary members of the Editorial Board. The journal encompasses all the varied aspects of entomological research. This has become the need felt in scientific research due to the emphasis on intra-, inter-, and multi-disciplinary approach.