2016 Volume 3 Issue 5

Delivery of drugs HSA protein with [Pd (bipy) Glycine] Cl complexes


Morteza Keshavarz
Abstract

Human serum albumin (HSA) is the most abundant protein in the circulatory system; HSA is an abundant plasma protein that binds a wide variety interaction of [Pd (bipy) Glycine] Cl. The complex [Pd (bipy) Glycine] Cl has been synthesized and purified and the spectral characteristics of this complex were determined and a series of titration experiments were designed with respect to titration of complex with HAS solution. These experiments are done at pH=7, 1mM phosphate buffer and at 20, 25, 30, 37 and 40 ºC. The titration spectra were analyzed at each temperature based on binding models of 1:1, 1:2 and 2:1, using SQUAD software .The results represents the formation of 1:1 complex between drug and HSA .All of the thermodynamic parameters such as Δ G , Δ H , Δ S and formation constant (K) were calculated with their uncertainty. These results were interpreted on the basis of molecular viewpoint and represent the endothermic of process. Hence the process is entropy driven; this confirms the rule of hydrophobic interaction in the formation of drug-HSA complex. This evidence is also certified by studying the effect of ionic strength on the UV-Vis spectra of drug-HSA.

 

Keywords: [Pd (bipy) Glycine] Cl; HAS; Ionic strength; UV-Vis spectra; Drug delivery


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Entomology and Applied Science Letters is an international peer reviewed publication which publishes scientific research & review articles related to insects that contain information of interest to a wider audience, e.g. papers bearing on the theoretical, genetic, agricultural, medical and biodiversity issues. Emphasis is also placed on the selection of comprehensive, revisionary or integrated systematics studies of broader biological or zoogeographical relevance. Papers on non-insect groups are no longer accepted. In addition to full-length research articles and reviews, the journal publishes interpretive articles in a Forum section, Short Communications, and Letters to the Editor. The journal publishes reports on all phases of medical entomology and medical acarology, including the systematics and biology of insects, acarines, and other arthropods of public health and veterinary significance.
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