Mitochondrial DNA CO1 gene sequence was used for the identification of Apis florae(A. florae) which is one of honey bees species, it is called dwarf honey bee as it is small size comparing to other types of honey bees, It makes a single comb on the tree, and was distributed in Asian countries, so it called Asian bees. The dwarf honey bee was recorded for the first time in Africa in 1987 and since that it was recorded in several countries, the dwarf bees are important pollinators. Accidentally A. florae introduced into Saudi Arabia, Jordan, Sudan, Ethiopia, and recently recorded in Egypt (Suez City). A. florae samples were screened for mitochondrial cytochrome oxidase I gene with PCR and the product was sequenced; Sequence of Egyptian Apis florae was analyzed and studied to determine the origin of these population. It was concluded that the Egyptian population was similar and identical with the Indian population by 99% - 100%, query 91-100%, these means that the Egyptian A. florae belong to the Indian species, came from the east, probably from Jordan and move into the Sinai Peninsula, then to Suez city. A sequence of Egyptian population A. florae which is the first record in Egypt was added to the gene bank with Accession No: (PRJNA556448).