Characterization and Molecular Identification of the Mexican Strain  CP-MABb1 of Beauveria sp

Luis J Amaro Leal; Omar Romero-Arenas; Alfredo Baez Simon; J. António Rivera Tapia; Conrado Parraguirre Lezama Parraguirre Lezama; Primo Sanchez Morales Sanchez Morales

2018 Volume 5 Issue 2

Characterization and Molecular Identification of the Mexican Strain CP-MABb1 of Beauveria sp

Luis J Amaro Leal, Omar Romero-Arenas, Alfredo Baez Simon, J. António Rivera Tapia, Conrado Parraguirre Lezama Parraguirre Lezama, Primo Sanchez Morales Sanchez Morales

Abstract

Biological control is used to reduce pest insect populations and is an alternative technique to the use of synthetic insecticides. The use of entomopathogenic fungi as microbial control, has advantages over chemical insecticides, among them its high specificity on the hosts and its low environmental pollution. However, the production of biological control agents such as Beauveria bassiana, presents limitations in the characterization and identification and selection of strains with high pathogenicity, likewise the potential that native strains can have for the management of insect pests in Mexico is unknown. The CP-MABb1 obtained a 99% homology for B. bassiana of the National Center of Biotechnology Information (NCBI), and presented the highest number of conidia and a viability of 53% in PDA culture medium, representing higher production of reproductive structures in comparison to the commercial strain, virulence determinants and selection in the biological control of pests.

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References

Bateman, R., Carey, M., Batt, D., Prior, C., Abraham, Y., Moore, D., Jenkins, N., Fenlon, J., Biocontrol Science and Technology, 1996. 6, 549-60, 1996.

Bateman, R., Carey, M., Moore, D., Prior, C., Annals of Applied Biology,1993. 122, 145-52.

Roy, H.E., Steinkraus, D.C., Eilenberg, A.E., Pell, J.K., Annual Review of Entomology, 2006. 51(1), 331-357.

Barajas, O.G., Morales, R.D., Del Pozo, N.E., Rodríguez, A.M., Nuñez, L.J., Revista Tecno-ciencia Chihuahua, 2009. 3(1), 33-38.

Rodríguez, L.A., Arredondo, H.C., In: Theory and Application of Biological Control. Mexi-can Society of Biological Control, Mexico, 2007. 303 p.

Polar, P., Moore, D., Kairo, M., Ramsubhag, A., Exp Appl Acarol., 2008. 46(1), 119-148.

Butt, T.M., Jackson, C., Magan, N., In: Fungi as Biocontrol Agents. Progress, Problems and Potential. CABI Publishing, Wallingford, Unit-ed Kingdom, 2001. 385 p.

White, T.J., Bruns, T., Lee, S., Taylor, J., In: PCR Protocols, a Guide to Methods and Appli-cations. Eds. M. A. Innis, D. H. Gelfand, J. J. Sninsky and T. J. White. Academic Press, San Diego, 1990. 315, 322 pp.

Tamura, K., Nei, M., Kumar, S., Proceedings of the National Academy of Sciences, 2004. 101, 11030-11035.

Tamura, K., Dudley, J., Nei, M., Kumar, S., Mo-lecular Biology and Evolution, 2007. 24, 1596-1599.

Benítez, N., In: Microbiology laboratory manual. Dept. of Biology. Universidad del Valle. Cali. Colombia, 2002. 147 pp.

Alean, CIEn: Bachelor thesis, Pontificia Uni-versidad Javeriana, Bogotá, Colombia, 2003. 120 pp.

Domsch, K.H., Gams, W., Anderson, T.H., In: Academic Press. Compendium of soil fungi. Ed. San Francisco, 1993. 672 p.

Glare, T.R., Inwood, A.J., Mycological Re-search, 1998. 102, 250-256.

Wada, S., Horita, M., Hirayae, K., Shimazu, M., Applied Entomology and Zoology, 2003. 38, 551-557.

Muro, M.A., Elliott, S., Moore, D., Parker, L.B., Skinner, M., Reid, W., Bouhssini, M., Myco-logical Research, 2005. 109, 294-306.

Cruz, L.P., Gaitan, A.L., Gongora, C.E., Applied Microbiology and Biotechnology, 2006. 71, 918-926.

Bartlett, M.C., Jaronski, S.T., In: Burge, M.N. (Ed.), Fungi in Biological Control Systems, Manchester University Press, Manchester, UK, 1988. 61-85 pp.

Feng, M.G., Poprawski, T.J., Khachatourians, G.G. 1994. Biocontrol Science and Technolo-gy, 1994. 4, 3-34.

Shah, F.A., Wang, C.S., Butt, T.M., FEMS Mi-crobiol. Lett., 2005. 251, 259-266.