2016 Volume 3 Issue 5

An In Vitro Study to Compare Antibacterial Efficacy of Various Root Canal Irrigants Used In Endodontics

Zeeshan Qamar, Tayyaba Fatima

The aim of the study was to compare antibacterial efficacy of various root canal irrigants used in department of endodontics. One hundred and fifty freshly extracted mandibular & maxillary molars were stored in normal saline at room temperatures .The teeth were deroofed & their wider canals that is distal canal in mandibular molars & palatal canal in maxillary molars were selected. Working lengths of specimens were recorded. All teeth were washed & autoclaved. Widening of canals was done using the K-file with a step back technique and divided into 5 groups (30/group).The teeth were then inoculated with 10 microliter of bacterial suspension of enterococcus faecalis fixed at 0.144 absorbance value (550nm wavelength) and incubated for 48-hrs. There was evident statistically significant difference in the antibacterial efficiency between 5% sodium hypochlorite (NaOCl) (positive control) (Group A) 0.12% chlorhexidine (CHX) (Group B), 2% Iodine (Iodine potassium iodide) (Group C), Sodium Chloride (NaCl) (Group D) and distilled water (Group E) (negative control) (ρ<0.05). Group A where 5ml of 5% NaOCl was used at initial & final rinse as root canal irrigants showed the maximum antibacterial efficiency against streptococci followed by Group B, Group C, Group D & Group E (Control Group).

Key Words: Root canal irrigants, NaOCl, chlorhexidine, NaCl, Iodine


Patel, S. and J.J. Barnes, Introduction, in The Principles of Endodontics. 2013, Oxford, UK. p. 1-5.

Samaksamarn, T., et al., Chulalongkorn University Dental Journal. 31(2): p. 125-34.

Pashley, E.L., et al., Cytotoxic effects of NaOCl on vital tissue. J Endod, 1985. 11(12): p. 525-8.

Dakin, H.D., British medical journal, 1915. 2(2852): p. 318.

Crane, A.B., A practicable root-canal technic. 1920: Lea & Febiger.

Best, M., V.S. Springthorpe, and S.A. Sattar, Am J Infect Control, 1994. 22(3): p. 152-62.

Underwood, M.A. and S. Pirwitz, Am J Infect Control, 1999. 27(2): p. 141-4.

Haapasalo, M., et al., Endodontic topics, 2005. 10(1): p. 77-102.

Schafer, E. and K. Bossmann, American Journal of Dentistry, 2001. 14(4): p. 233-237.

Krautheim, A.B., T.H.M. Jermann, and A.J. Bircher, Contact Dermatitis, 2004. 50(3): p. 113-116.

Yesilsoy, C., et al., Journal of Endodontics, 1995. 21(10): p. 513-515.

Southard, S.R., et al., Journal of periodontology, 1989. 60(6): p. 302-309.

Shih, M., F.J. Marshall, and S. Rosen, Oral Surgery, Oral Medicine, Oral Pathology, 1970. 29(4): p. 613-619.

Ohara, P., M. Torabinejad, and J.D. Kettering, Endodontics & Dental Traumatology, 1993. 9(3): p. 95-100.

Thrower, Y., R.J. Pinney, and M. Wilson, Journal of Medical Microbiology, 1997. 46(5): p. 425-429.

Desai, M., et al., Journal of Antimicrobial Chemotherapy, 1998. 42(2): p. 153-160.

D'Arcangelo, C., G. Varvara, and P. De Fazio, Journal of Endodontics, 1999. 25(5): p. 351-353.

Wilson, M., Journal of Medical Microbiology, 1996. 44(2): p. 79-87.

Pratten, J. and M. Wilson, Antimicrobial Agents and Chemotherapy, 1999. 43(7): p. 1595-1599.

Siqueira, J.F., et al., Journal of Endodontics, 1998. 24(6): p. 414-416.

Briseno, B.M., et al., Endodontics & Dental Traumatology, 1992. 8(1): p. 6-11.

Siren, E.K., et al., International Endodontic Journal, 1997. 30(2): p. 91-95.

Grahnen, H. and B. Krasse, Odontol Rev, 1963. 14: p. 167-177.

Olgart, L.G., Acta Odontol Scand, 1969. 27(1): p. 91-103.

Myers, J.W., F.J. Marshall, and S. Rosen, Oral Surgery, Oral Medicine, Oral Pathology, 1969. 28(6): p. 889-896.

Bystrom, A. and G. Sundqvist, Oral Surgery Oral Medicine Oral Pathology Oral Radiology and Endodontics, 1983. 55(3): p. 307-312.

Bystrom, A. and G. Sundqvist, International Endodontic Journal, 1985. 18(1): p. 35-40.

Gomes, B.P.F.A., J.D. Lilley, and D.B. Drucker, International Endodontic Journal, 1996. 29(4): p. 235-241.

Molander, A., C. Reit, and G. Dahlén, Dental Traumatology, 1999. 15(5): p. 205-209.

Delany, G.M., et al., Oral Surgery Oral Medicine Oral Pathology Oral Radiology and Endodontics, 1982. 53(5): p. 518-523.

Jeansonne, M.J. and R.R. White, Journal of Endodontics, 1994. 20(6): p. 276-278.

Ringel, A.M., et al., Journal of Endodontics, 1982. 8(5): p. 200-204.

Karuvilla, J.R. and M.P. Kamath, Journal of Endodontics, 1998. 24(7): p. 472-476.

Leonardo, M.R., et al., Journal of Endodontics, 1999. 25(3): p. 167-171.

Entomology and Applied Science Letters is an international peer reviewed publication which publishes scientific research & review articles related to insects that contain information of interest to a wider audience, e.g. papers bearing on the theoretical, genetic, agricultural, medical and biodiversity issues. Emphasis is also placed on the selection of comprehensive, revisionary or integrated systematics studies of broader biological or zoogeographical relevance. Papers on non-insect groups are no longer accepted. In addition to full-length research articles and reviews, the journal publishes interpretive articles in a Forum section, Short Communications, and Letters to the Editor. The journal publishes reports on all phases of medical entomology and medical acarology, including the systematics and biology of insects, acarines, and other arthropods of public health and veterinary significance.
Issue 2 Volume 7 - 2020
Call for Papers
Entomology and Applied Science Letters supports the submission of entomological papers that contain information of interest to a wider reader groups e. g. papers bearing on taxonomy, phylogeny, biodiversity, ecology, systematic, agriculture, morphology. The selection of comprehensive, revisionary or integrated systematics studies of broader biological or zoogeographical relevance is also important. Distinguished entomologists drawn from different parts of the world serve as honorary members of the Editorial Board. The journal encompasses all the varied aspects of entomological research. This has become the need felt in scientific research due to the emphasis on intra-, inter-, and multi-disciplinary approach.